Phylogenetic clades 6 and 8 of enterohemorrhagic Escherichia coli O157: H7 With particular stx subtypes are more frequently found in isolates from hemolytic uremic syndrome patients than from asymptomatic carriers

EHEC Working Group in Japan

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24 Citations (Scopus)

Abstract

Background. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined. Methods. Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999-2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation. Results. Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/ or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0-9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact norV gene, which encodes for a nitric oxide reductase that inhibits Shiga toxin activity under anaerobic condition, in all clades 1-3 isolates but not in clades 4-8 isolates. Conclusions. Early detection of EHEC O157:H7 strains that belonged to clades 6/8 and harbored specific stx subtypes may be important for defining the risk of disease progression in EHEC-infected 0- to 9-year-old children.

Original languageEnglish
JournalOpen Forum Infectious Diseases
Volume1
Issue number2
DOIs
Publication statusPublished - Jan 1 2014

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Enterohemorrhagic Escherichia coli
Hemolytic-Uremic Syndrome
Escherichia coli O157
Shiga Toxin
Pulsed Field Gel Electrophoresis
Disease Progression
Diarrhea
Japan
Logistic Models
Regression Analysis
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Clinical Neurology

Cite this

@article{c169f1f269f348ad9beeb8df132cc0af,
title = "Phylogenetic clades 6 and 8 of enterohemorrhagic Escherichia coli O157: H7 With particular stx subtypes are more frequently found in isolates from hemolytic uremic syndrome patients than from asymptomatic carriers",
abstract = "Background. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined. Methods. Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999-2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation. Results. Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/ or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0-9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact norV gene, which encodes for a nitric oxide reductase that inhibits Shiga toxin activity under anaerobic condition, in all clades 1-3 isolates but not in clades 4-8 isolates. Conclusions. Early detection of EHEC O157:H7 strains that belonged to clades 6/8 and harbored specific stx subtypes may be important for defining the risk of disease progression in EHEC-infected 0- to 9-year-old children.",
author = "{EHEC Working Group in Japan} and Sunao Iyoda and Manning, {Shannon D.} and Kazuko Seto and Keiko Kimata and Junko Isobe and Yoshiki Etoh and Sachiko Ichihara and Yuji Migita and Kikuyo Ogata and Mikiko Honda and Tsutomu Kubota and Kimiko Kawano and Kazutoshi Matsumoto and Jun Kudaka and Norio Asai and Junko Yabata and Kiyoshi Tominaga and Jun Terajima and Tomoko Morita-Ishihara and Hidemasa Izumiya and Yoshitoshi Ogura and Takehito Saitoh and Atsushi Iguchi and Hideki Kobayashi and Yukiko Hara-Kudo and Makoto Ohnishi and Kazumi Horikawa and Nanami Asoshima and Mitsuhiro Kameyama and Reiko Arai and Masao Kawase and Yukiko Asano and Kazuki Chiba and Ichiro Furukawa and Toshiro Kuroki and Madoka Hamada and Seiya Harada and Takashi Hatakeyama and Takashi Hirochi and Yumiko Sakamoto and Midori Hiroi and Takashi Kanda and Kaori Iwabuchi and Hitomi Kasahara and Shinya Kawanishi and Koji Kikuchi and Hiroyuki Ueno and Tomoko Kitahashi and Yuka Kojima and Noriko Konishi",
year = "2014",
month = "1",
day = "1",
doi = "10.1093/ofid/ofu061",
language = "English",
volume = "1",
journal = "Open Forum Infectious Diseases",
issn = "2328-8957",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Phylogenetic clades 6 and 8 of enterohemorrhagic Escherichia coli O157

T2 - H7 With particular stx subtypes are more frequently found in isolates from hemolytic uremic syndrome patients than from asymptomatic carriers

AU - EHEC Working Group in Japan

AU - Iyoda, Sunao

AU - Manning, Shannon D.

AU - Seto, Kazuko

AU - Kimata, Keiko

AU - Isobe, Junko

AU - Etoh, Yoshiki

AU - Ichihara, Sachiko

AU - Migita, Yuji

AU - Ogata, Kikuyo

AU - Honda, Mikiko

AU - Kubota, Tsutomu

AU - Kawano, Kimiko

AU - Matsumoto, Kazutoshi

AU - Kudaka, Jun

AU - Asai, Norio

AU - Yabata, Junko

AU - Tominaga, Kiyoshi

AU - Terajima, Jun

AU - Morita-Ishihara, Tomoko

AU - Izumiya, Hidemasa

AU - Ogura, Yoshitoshi

AU - Saitoh, Takehito

AU - Iguchi, Atsushi

AU - Kobayashi, Hideki

AU - Hara-Kudo, Yukiko

AU - Ohnishi, Makoto

AU - Horikawa, Kazumi

AU - Asoshima, Nanami

AU - Kameyama, Mitsuhiro

AU - Arai, Reiko

AU - Kawase, Masao

AU - Asano, Yukiko

AU - Chiba, Kazuki

AU - Furukawa, Ichiro

AU - Kuroki, Toshiro

AU - Hamada, Madoka

AU - Harada, Seiya

AU - Hatakeyama, Takashi

AU - Hirochi, Takashi

AU - Sakamoto, Yumiko

AU - Hiroi, Midori

AU - Kanda, Takashi

AU - Iwabuchi, Kaori

AU - Kasahara, Hitomi

AU - Kawanishi, Shinya

AU - Kikuchi, Koji

AU - Ueno, Hiroyuki

AU - Kitahashi, Tomoko

AU - Kojima, Yuka

AU - Konishi, Noriko

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined. Methods. Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999-2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation. Results. Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/ or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0-9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact norV gene, which encodes for a nitric oxide reductase that inhibits Shiga toxin activity under anaerobic condition, in all clades 1-3 isolates but not in clades 4-8 isolates. Conclusions. Early detection of EHEC O157:H7 strains that belonged to clades 6/8 and harbored specific stx subtypes may be important for defining the risk of disease progression in EHEC-infected 0- to 9-year-old children.

AB - Background. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined. Methods. Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999-2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation. Results. Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/ or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0-9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact norV gene, which encodes for a nitric oxide reductase that inhibits Shiga toxin activity under anaerobic condition, in all clades 1-3 isolates but not in clades 4-8 isolates. Conclusions. Early detection of EHEC O157:H7 strains that belonged to clades 6/8 and harbored specific stx subtypes may be important for defining the risk of disease progression in EHEC-infected 0- to 9-year-old children.

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U2 - 10.1093/ofid/ofu061

DO - 10.1093/ofid/ofu061

M3 - Article

AN - SCOPUS:84942513698

VL - 1

JO - Open Forum Infectious Diseases

JF - Open Forum Infectious Diseases

SN - 2328-8957

IS - 2

ER -