Phylogenetic diversity of indigenous cowpea bradyrhizobia from soils in Japan based on sequence analysis of the 16S-23S rRNA internal transcribed spacer (ITS) region

Papa Saliou Sarr, Takeo Yamakawa, Yuichi Saeki, Aliou Guisse

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cowpea [. Vigna unguiculata (L.) Walp.] is an important legume crop and yet its rhizobia have not been well characterized in many areas. In the present study, sequence analysis of the bacterial 16S-23S rRNA internal transcribed spacer (ITS) region was performed to characterize genetically 76 indigenous cowpea rhizobia from five different geographic regions (Okinawa, Miyazaki, Kyoto, Fukushima and Hokkaido) of Japan. The sequence analysis clustered all isolates in the genus Bradyrhizobium. They were conspecific with B. japonicum, B. yuanmingense, B. elkanii and Bradyrhizobium sp., although none of them grouped with B. liaoningense, B. canariense, B. betae or B. iriomotense. B. yuanmingense was only isolated from the southern region (Okinawa) where it achieved the highest frequency of 69%. B. japonicum was predominant at Miyazaki, Fukushima and Hokkaido with more than 60% of the isolates. B. elkanii was mainly recorded in the southern (Okinawa: 31%, Miyazaki: 33%) and middle (Kyoto: 33%) regions. This species was present at a very low frequency in Fukushima and absent in Hokkaido in the northern area. Bradyrhizobium sp. like-strains were absent in the southern part (Okinawa, Miyazaki) but were concentrated either in the middle regions with 67% of Kyoto isolates and 28% of Fukushima isolates, and in the northern region with 40% of the Hokkaido isolates. This study revealed a geographical distribution of cowpea bradyrhizobia which seemed to be related to the differences in the environmental characteristics (soil type and soil pH, temperature, climate, moisture) of the different regions in Japan.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalSystematic and Applied Microbiology
Volume34
Issue number4
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Bradyrhizobiaceae
Bradyrhizobium
cowpeas
internal transcribed spacers
Sequence Analysis
Japan
Soil
sequence analysis
Ryukyu Archipelago
ribosomal RNA
phylogenetics
Rhizobium
phylogeny
soil
rhizobacterium
Bradyrhizobium liaoningense
Climate
Bradyrhizobium elkanii
Fabaceae
Bradyrhizobium japonicum

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

Phylogenetic diversity of indigenous cowpea bradyrhizobia from soils in Japan based on sequence analysis of the 16S-23S rRNA internal transcribed spacer (ITS) region. / Sarr, Papa Saliou; Yamakawa, Takeo; Saeki, Yuichi; Guisse, Aliou.

In: Systematic and Applied Microbiology, Vol. 34, No. 4, 01.06.2011, p. 285-292.

Research output: Contribution to journalArticle

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abstract = "Cowpea [. Vigna unguiculata (L.) Walp.] is an important legume crop and yet its rhizobia have not been well characterized in many areas. In the present study, sequence analysis of the bacterial 16S-23S rRNA internal transcribed spacer (ITS) region was performed to characterize genetically 76 indigenous cowpea rhizobia from five different geographic regions (Okinawa, Miyazaki, Kyoto, Fukushima and Hokkaido) of Japan. The sequence analysis clustered all isolates in the genus Bradyrhizobium. They were conspecific with B. japonicum, B. yuanmingense, B. elkanii and Bradyrhizobium sp., although none of them grouped with B. liaoningense, B. canariense, B. betae or B. iriomotense. B. yuanmingense was only isolated from the southern region (Okinawa) where it achieved the highest frequency of 69{\%}. B. japonicum was predominant at Miyazaki, Fukushima and Hokkaido with more than 60{\%} of the isolates. B. elkanii was mainly recorded in the southern (Okinawa: 31{\%}, Miyazaki: 33{\%}) and middle (Kyoto: 33{\%}) regions. This species was present at a very low frequency in Fukushima and absent in Hokkaido in the northern area. Bradyrhizobium sp. like-strains were absent in the southern part (Okinawa, Miyazaki) but were concentrated either in the middle regions with 67{\%} of Kyoto isolates and 28{\%} of Fukushima isolates, and in the northern region with 40{\%} of the Hokkaido isolates. This study revealed a geographical distribution of cowpea bradyrhizobia which seemed to be related to the differences in the environmental characteristics (soil type and soil pH, temperature, climate, moisture) of the different regions in Japan.",
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