Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences

Yukiko Maeda, Hirosuke Shinohara, Akinori Kiba, Kouhei Ohnishi, Naruto Furuya, Yoshiaki Kawamura, Takayuki Ezaki, Peter Vandamme, Seiya Tsushima, Yasufumi Hikichi

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

In order to develop a detection method for the rice pathogens Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli, the phylogeny of six plant-pathogenic Burkholderia species was analysed using the combined nucleotide sequences of gyrB and rpoD. B. plantarii, B. glumae and B. gladioli formed tight monophyletic branches supported by high bootstrap probabilities. The high sequence similarity revealed a close phylogenetic relationship between B. glumae and B. plantarii. B. plantarii strains were divided into three subclusters comprising rice strains, whereas the single Vanda strain occupied a unique position in the phylogenetic tree. The gyrB and rpoD sequences of all a glumae strains examined were highly conserved. In contrast, B. gladioli strains demonstrated a far greater sequence diversity, but this diversity did not correlate with pathovar, host plant or geographical origin of the strains. A multiplex-PCR protocol using specific primers from the gyrB sequences was designed that allowed the specific detection and identification of B. plantarii, B. glumae and B. gladioli in rice seeds infected with these pathogenic species.

Original languageEnglish
Article number64184
Pages (from-to)1031-1038
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number5
DOIs
Publication statusPublished - May 2006

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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