Phylogeography of Eurasian Larix species inferred from nucleotide variation in two nuclear genes

Ismael A. Khatab, Hiroko Ishiyama, Nobuyuki Inomata, Xiao Ru Wang, Alfred Szmidt

Research output: Contribution to journalArticle

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Abstract

Larch (Larix Mill.) is one of the most widely distributed tree genera in Eurasia. To determine population structure and to verify classification of five species and three varieties of the Eurasian Larix species, we investigated levels and patterns of nucleotide variation of two nuclear gene regions: the 4-coumarate coenzyme A ligase (4CL) and the coumarate 3-hydroxylase (C3H). In the 4CL region nucleotide diversity at silent sites (φsil) varied between 0.0020 in L. gmelinii to 0.0116 in L. gmelinii var. japonica and in the C3H region between 0.0019 in L. kaempferi to 0.0066 in L. gmelinii var. japonica. In both gene regions statistically significant population differentiation (FST) was detected among adjacent refugial populations of some species suggesting limited gene flow and/or long time isolation of some refugial populations. On the other hand, populations of L. sukaczewii from northwestern Russia, which was glaciated 20,000 years ago showed no differentiation. This result is consistent with recent postglacial origin of these populations. Haplotype composition of some of the investigated Eurasian Larix species suggested that they are considerably diverged. Some haplotypes were unique to individual species. Our results indicate that more intensive sampling especially from known refugial regions is necessary for inferring correct classification of Eurasian Larix species and inferring their postglacial migration.

Original languageEnglish
Pages (from-to)55-66
Number of pages12
JournalGenes and Genetic Systems
Volume83
Issue number1
DOIs
Publication statusPublished - Apr 18 2008

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Larix
Phylogeography
Nucleotides
Coenzyme A Ligases
Population
Genes
Mixed Function Oxygenases
Haplotypes
Gene Flow
Russia

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Phylogeography of Eurasian Larix species inferred from nucleotide variation in two nuclear genes. / Khatab, Ismael A.; Ishiyama, Hiroko; Inomata, Nobuyuki; Wang, Xiao Ru; Szmidt, Alfred.

In: Genes and Genetic Systems, Vol. 83, No. 1, 18.04.2008, p. 55-66.

Research output: Contribution to journalArticle

Khatab, Ismael A. ; Ishiyama, Hiroko ; Inomata, Nobuyuki ; Wang, Xiao Ru ; Szmidt, Alfred. / Phylogeography of Eurasian Larix species inferred from nucleotide variation in two nuclear genes. In: Genes and Genetic Systems. 2008 ; Vol. 83, No. 1. pp. 55-66.
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