Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci

Kozue Oana, Yukie Okimura, Yoshiyuki Kawakami, Nobuaki Hayashida, Makoto Shimosaka, Mitsuo Okazaki, Tetsuya Hayashi, Makoto Ohnishi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three additional E. faecium strains were also analyzed by I-CeuI digestion, and the genome sizes were found to vary from 2550 to 2995 kb. We further investigated the genome sizes and number of rrn operons in four E. faecalis, one E. avium, and one E. durans strains. The genome sizes were larger than E. faecium: 3000-3250 kb in E. faecalis, 3445 kb in E. avium, and 3070 kb in E. durans. E. avium and E. durans contained six rrn operons as in E. faecium, but all the E. faecalis strains possessed four rrn operons.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalFEMS microbiology letters
Volume207
Issue number2
DOIs
Publication statusPublished - Feb 5 2002
Externally publishedYes

Fingerprint

Enterococcus faecium
Enterococcus
Operon
Genome Size
rRNA Operon
Chromosomes
Circular DNA
Digestion
Genome

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci. / Oana, Kozue; Okimura, Yukie; Kawakami, Yoshiyuki; Hayashida, Nobuaki; Shimosaka, Makoto; Okazaki, Mitsuo; Hayashi, Tetsuya; Ohnishi, Makoto.

In: FEMS microbiology letters, Vol. 207, No. 2, 05.02.2002, p. 133-139.

Research output: Contribution to journalArticle

Oana, Kozue ; Okimura, Yukie ; Kawakami, Yoshiyuki ; Hayashida, Nobuaki ; Shimosaka, Makoto ; Okazaki, Mitsuo ; Hayashi, Tetsuya ; Ohnishi, Makoto. / Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci. In: FEMS microbiology letters. 2002 ; Vol. 207, No. 2. pp. 133-139.
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