Physical properties of the extracellular matrix of decellularized porcine liver

Hiroyuki Ijima, Shintaro Nakamura, Ronald Bual, Nana Shirakigawa, Shuichi Tanoue

Research output: Contribution to journalArticlepeer-review

Abstract

The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical properties of L-ECM and L-ECM gels. L-ECM was prepared as a liver-specific matrix substrate from solubilized decellularized porcine liver. In comparison to type I collagen, L-ECM yielded a lower elasticity and exhibited an abrupt decrease in its elastic modulus at 37 C. Its elastic modulus increased at increased temperatures, and the storage elastic modulus value never fell below the loss modulus value. An increase in the gel concentration of L-ECM resulted in a decrease in the biodegradation rate and in an increase in mechanical strength. The reported properties of L-ECM gel (10 mg/mL) were equivalent to those of collagen gel (3 mg/mL), which is commonly used in regenerative medicine and gel cultures. Based on reported findings, the physical properties of the novel functional substrate for culturing and regenerative medicine L-ECM were quantified.

Original languageEnglish
Article number39
JournalGels
Volume4
Issue number2
DOIs
Publication statusPublished - Jun 2018

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Polymers and Plastics

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