Physiological difference between free and triglyceride-type conjugated linoleic acid on the immune function of C57BL/6N mice

Masao Yamasaki, Takae Kitagawa, Hitomi Chujo, Nami Koyanagi, Eri Nishida, Mako Nakaya, Kazuma Yoshimi, Hidenori Maeda, Shinsuke Nou, Toshio Iwata, Kanehide Ogita, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Previous studies have shown the physiological significance of dietary conjugated linoleic acid (CLA) in various experimental animals and in human beings. One of the important problems to better elucidate is the difference between triglyceride (TG) and free (FFA) dietary CLA. Here, using splenocytes, this study assesses how TG- and FFA-CLA modulate immunoglobulin and various cytokine productions. In this study, C57BL/6N mice were fed an experimental diet containing 0% CLA, 0.1 or 1% FFA-CLA, or 0.1 or 1% TG-CLA for 3 weeks. The production of immunoglobulin tended to be up-regulated by 1% FFA-CLA. As a result of protein array analysis using the supernatant from splenocytes cultured with no CLA, 1% FFA-CLA, and TG-CLA, some cytokine production was shown to be remarkably regulated by dietary FFA- and TG-CLA. A total of 32 cytokines were examined, and 11-14 produced cytokines that were 2-fold up-regulated as compared with control for FFA- or TG-CLA, respectively. Especially, the production of IL-9 and MCP-5 and other cytokines was remarkably up-regulated by both FFA- and TG-CLA. In addition, seven cytokines were 2-fold down-regulated by TG-CLA. These data show that there is a slight but significant difference between the functionalities of FFA- and TG-CLA.

Original languageEnglish
Pages (from-to)3644-3648
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number11
DOIs
Publication statusPublished - Jun 2 2004

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Conjugated Linoleic Acids
conjugated linoleic acid
Inbred C57BL Mouse
Triglycerides
triacylglycerols
mice
cytokines
Cytokines
splenocytes
immunoglobulins
Immunoglobulins
interleukin-9
Interleukin-9
Protein Array Analysis
Nutrition

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Physiological difference between free and triglyceride-type conjugated linoleic acid on the immune function of C57BL/6N mice. / Yamasaki, Masao; Kitagawa, Takae; Chujo, Hitomi; Koyanagi, Nami; Nishida, Eri; Nakaya, Mako; Yoshimi, Kazuma; Maeda, Hidenori; Nou, Shinsuke; Iwata, Toshio; Ogita, Kanehide; Tachibana, Hirofumi; Yamada, Koji.

In: Journal of Agricultural and Food Chemistry, Vol. 52, No. 11, 02.06.2004, p. 3644-3648.

Research output: Contribution to journalArticle

Yamasaki, M, Kitagawa, T, Chujo, H, Koyanagi, N, Nishida, E, Nakaya, M, Yoshimi, K, Maeda, H, Nou, S, Iwata, T, Ogita, K, Tachibana, H & Yamada, K 2004, 'Physiological difference between free and triglyceride-type conjugated linoleic acid on the immune function of C57BL/6N mice', Journal of Agricultural and Food Chemistry, vol. 52, no. 11, pp. 3644-3648. https://doi.org/10.1021/jf049929j
Yamasaki, Masao ; Kitagawa, Takae ; Chujo, Hitomi ; Koyanagi, Nami ; Nishida, Eri ; Nakaya, Mako ; Yoshimi, Kazuma ; Maeda, Hidenori ; Nou, Shinsuke ; Iwata, Toshio ; Ogita, Kanehide ; Tachibana, Hirofumi ; Yamada, Koji. / Physiological difference between free and triglyceride-type conjugated linoleic acid on the immune function of C57BL/6N mice. In: Journal of Agricultural and Food Chemistry. 2004 ; Vol. 52, No. 11. pp. 3644-3648.
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AU - Nishida, Eri

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