Physiological study of the wild edible mushroom leucocalocybe mongolica

Tie Lu, Tolgor Bau, Shoji Ohga

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Leucocalocybe mongolica (S. Imai) X.D. Yu & Y.J. Yao, a well-known edible and medicinal mushroom is endangered as its habitat is continually under threat from human activities, which has been lessening year after year. In this paper, the biological characteristics of the fungus of strain isolated from the wild fruit body were studied. For the screening of single optimal factor, carbon sources, nitrogen sources, inorganic salt and growth factor were tested for the mycelia growth rate. As a result, the fungus grew better on maltose, starch and cellobiose as the carbon sources; yeast extract, beef powder, beef extract as the nitrogen sources; K2HPO4 Fe2(SO4)3 as the inorganic salt; some decoctions such as of Pleurotus ostreatus, carrot, soybean sprouts as the growth factor. The orthogonal experiments with the above selected three conditions for each of the four factors were then performed and the optimal cultivation conditions were determined. A descending order of the impact for the four factors was growth factor > inorganic salt > nitrogen source > carbon source and the F-Test showed very significant difference among all the four factors. The optimal culture condition for L. mongolica was the combination of starch and beef extract, decoction of 2% carrot, no added inorganic salt. Based on this conclusion of the orthogonal experiment, the optimal medium in mycelium cultures was applied to temperature and pH experiments, anointed with pH=6.5 and at 25°C were suggested that good growth of mushrooms.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume62
Issue number1
Publication statusPublished - Feb 1 2017

Fingerprint

wild edible mushrooms
inorganic salts
Agaricales
Salts
beef extracts
growth factors
Intercellular Signaling Peptides and Proteins
Daucus carota
Nitrogen
Carbon
Mycelium
Starch
mushrooms
carrots
mycelium
carbon
Fungi
nitrogen
starch
Pleurotus

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Physiological study of the wild edible mushroom leucocalocybe mongolica. / Lu, Tie; Bau, Tolgor; Ohga, Shoji.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 62, No. 1, 01.02.2017, p. 1-8.

Research output: Contribution to journalArticle

@article{b5c0be1e5eaf492aa7b1f61e5baca2b7,
title = "Physiological study of the wild edible mushroom leucocalocybe mongolica",
abstract = "Leucocalocybe mongolica (S. Imai) X.D. Yu & Y.J. Yao, a well-known edible and medicinal mushroom is endangered as its habitat is continually under threat from human activities, which has been lessening year after year. In this paper, the biological characteristics of the fungus of strain isolated from the wild fruit body were studied. For the screening of single optimal factor, carbon sources, nitrogen sources, inorganic salt and growth factor were tested for the mycelia growth rate. As a result, the fungus grew better on maltose, starch and cellobiose as the carbon sources; yeast extract, beef powder, beef extract as the nitrogen sources; K2HPO4 Fe2(SO4)3 as the inorganic salt; some decoctions such as of Pleurotus ostreatus, carrot, soybean sprouts as the growth factor. The orthogonal experiments with the above selected three conditions for each of the four factors were then performed and the optimal cultivation conditions were determined. A descending order of the impact for the four factors was growth factor > inorganic salt > nitrogen source > carbon source and the F-Test showed very significant difference among all the four factors. The optimal culture condition for L. mongolica was the combination of starch and beef extract, decoction of 2{\%} carrot, no added inorganic salt. Based on this conclusion of the orthogonal experiment, the optimal medium in mycelium cultures was applied to temperature and pH experiments, anointed with pH=6.5 and at 25°C were suggested that good growth of mushrooms.",
author = "Tie Lu and Tolgor Bau and Shoji Ohga",
year = "2017",
month = "2",
day = "1",
language = "English",
volume = "62",
pages = "1--8",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "1",

}

TY - JOUR

T1 - Physiological study of the wild edible mushroom leucocalocybe mongolica

AU - Lu, Tie

AU - Bau, Tolgor

AU - Ohga, Shoji

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Leucocalocybe mongolica (S. Imai) X.D. Yu & Y.J. Yao, a well-known edible and medicinal mushroom is endangered as its habitat is continually under threat from human activities, which has been lessening year after year. In this paper, the biological characteristics of the fungus of strain isolated from the wild fruit body were studied. For the screening of single optimal factor, carbon sources, nitrogen sources, inorganic salt and growth factor were tested for the mycelia growth rate. As a result, the fungus grew better on maltose, starch and cellobiose as the carbon sources; yeast extract, beef powder, beef extract as the nitrogen sources; K2HPO4 Fe2(SO4)3 as the inorganic salt; some decoctions such as of Pleurotus ostreatus, carrot, soybean sprouts as the growth factor. The orthogonal experiments with the above selected three conditions for each of the four factors were then performed and the optimal cultivation conditions were determined. A descending order of the impact for the four factors was growth factor > inorganic salt > nitrogen source > carbon source and the F-Test showed very significant difference among all the four factors. The optimal culture condition for L. mongolica was the combination of starch and beef extract, decoction of 2% carrot, no added inorganic salt. Based on this conclusion of the orthogonal experiment, the optimal medium in mycelium cultures was applied to temperature and pH experiments, anointed with pH=6.5 and at 25°C were suggested that good growth of mushrooms.

AB - Leucocalocybe mongolica (S. Imai) X.D. Yu & Y.J. Yao, a well-known edible and medicinal mushroom is endangered as its habitat is continually under threat from human activities, which has been lessening year after year. In this paper, the biological characteristics of the fungus of strain isolated from the wild fruit body were studied. For the screening of single optimal factor, carbon sources, nitrogen sources, inorganic salt and growth factor were tested for the mycelia growth rate. As a result, the fungus grew better on maltose, starch and cellobiose as the carbon sources; yeast extract, beef powder, beef extract as the nitrogen sources; K2HPO4 Fe2(SO4)3 as the inorganic salt; some decoctions such as of Pleurotus ostreatus, carrot, soybean sprouts as the growth factor. The orthogonal experiments with the above selected three conditions for each of the four factors were then performed and the optimal cultivation conditions were determined. A descending order of the impact for the four factors was growth factor > inorganic salt > nitrogen source > carbon source and the F-Test showed very significant difference among all the four factors. The optimal culture condition for L. mongolica was the combination of starch and beef extract, decoction of 2% carrot, no added inorganic salt. Based on this conclusion of the orthogonal experiment, the optimal medium in mycelium cultures was applied to temperature and pH experiments, anointed with pH=6.5 and at 25°C were suggested that good growth of mushrooms.

UR - http://www.scopus.com/inward/record.url?scp=85020826670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020826670&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85020826670

VL - 62

SP - 1

EP - 8

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 1

ER -