Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis

Jukrapun Komaikul, Tharita Kitisripanya, Chadathorn Inyai, Kittisak Likhitwitayawuid, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

Abstract

Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the major bioactive stilbenes. The cell obtained from air-driven bioreactors such as round bottom, flat bottom, and air-lift vessel shape bioreactors was collected and analyzed for the levels of mulberroside A and oxyresveratrol. The results showed that the cell culture in round bottom and air-lift vessel bioreactors had higher growth rate, as compared with the cell culture in shake flasks (1.38- and 1.41-fold, respectively). The optimized culture condition to produce mulberroside A was obtained from round bottom bioreactor culture (55.56 ± 11.41 μmol/L). Additionally, endogenous stilbenoid hydrolysis of cell from the bioreactor culture was examined. Under optimized hydrolytic conditions, mulberroside A in the cell was readily deglycosylated to give oxyresveratrol within 1 h. These results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis. Interaction of the stilbenoid components with the endogenous hydrolytic enzyme triggered by cell disruption in M. alba samples was suggested to be the major cause of the alteration of the stilbenoid levels. These findings have provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture.

Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume55
Issue number2
DOIs
Publication statusPublished - Apr 15 2019

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Morus
Morus alba
Bioreactors
enzymatic hydrolysis
bioreactors
cell culture
Hydrolysis
Cell Culture Techniques
stilbenoids
Air
cells
air
Stilbenes
Morus (Moraceae)
stilbenes
mulberries
Glycosides
glycosides
raw materials
bioactive properties

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Plant Science

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Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis. / Komaikul, Jukrapun; Kitisripanya, Tharita; Inyai, Chadathorn; Likhitwitayawuid, Kittisak; Sritularak, Boonchoo; Tanaka, Hiroyuki; Putalun, Waraporn.

In: In Vitro Cellular and Developmental Biology - Plant, Vol. 55, No. 2, 15.04.2019, p. 199-208.

Research output: Contribution to journalArticle

Komaikul, Jukrapun ; Kitisripanya, Tharita ; Inyai, Chadathorn ; Likhitwitayawuid, Kittisak ; Sritularak, Boonchoo ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis. In: In Vitro Cellular and Developmental Biology - Plant. 2019 ; Vol. 55, No. 2. pp. 199-208.
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