Pig testis extract augments adiponectin expression and secretion through the peroxisome proliferator-activated receptor signaling pathway in 3T3-L1 adipocytes

Keishi Kadooka, Mikako Sato, Takashi Matsumoto, Satoru Kuhara, Yoshinori Katakura, Tatsuya Fujimura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the current study, we investigated whether hot-water extracts of specific livestock by-products induce the expression of adiponectin in mouse 3T3-L1 adipocytes. Out of the 11 extracts tested, pig testis extract (PTE) was found to enhance adiponectin expression and secretion by 3T3-L1 cells. Furthermore, simultaneous treatment with PTE and daidzein, a soy phytoestrogen, synergistically enhanced adiponectin secretion. Moreover, pretreatment with an estrogen receptor β antagonist (PHTPP) diminished adiponectin secretion from daidzein-treated cells but not from PTE-treated cells. Transcriptome analyses revealed that both daidzein and PTE regulate the peroxisome proliferator-activated receptor signaling pathway, although differences in the regulation of gene expression were observed between PTE- and daidzein-treated cells. These results suggest that PTE ameliorates lipid metabolic dysfunction by promoting adipocyte differentiation and enhancing adiponectin secretion via a mechanism different from that of daidzein.

Original languageEnglish
Pages (from-to)983-992
Number of pages10
JournalCytotechnology
Volume70
Issue number3
DOIs
Publication statusPublished - Jun 1 2018

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Peroxisome Proliferator-Activated Receptors
Adiponectin
Adipocytes
Testis
Swine
Cells
Medical problems
Gene expression
Agriculture
Lipids
Byproducts
Animals
Lipid Metabolism Disorders
3T3-L1 Cells
Plant Structures
Phytoestrogens
Molecules
Gene Expression Regulation
Livestock
Gene Expression Profiling

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Pig testis extract augments adiponectin expression and secretion through the peroxisome proliferator-activated receptor signaling pathway in 3T3-L1 adipocytes. / Kadooka, Keishi; Sato, Mikako; Matsumoto, Takashi; Kuhara, Satoru; Katakura, Yoshinori; Fujimura, Tatsuya.

In: Cytotechnology, Vol. 70, No. 3, 01.06.2018, p. 983-992.

Research output: Contribution to journalArticle

Kadooka, Keishi ; Sato, Mikako ; Matsumoto, Takashi ; Kuhara, Satoru ; Katakura, Yoshinori ; Fujimura, Tatsuya. / Pig testis extract augments adiponectin expression and secretion through the peroxisome proliferator-activated receptor signaling pathway in 3T3-L1 adipocytes. In: Cytotechnology. 2018 ; Vol. 70, No. 3. pp. 983-992.
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