Pigment composition patterns and expression analysis of flavonoid biosynthesis genes in the petals of evergreen azalea 'Oomurasaki' and its red flower sport

D. Mizuta, A. Nakatsuka, I. Miyajima, T. Ban, N. Kobayashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

With 2 figures and 3 tables To obtain basic information about flower colour sports in azalea breeding, the petals of Rhododendron × pulchrum'Oomurasaki' and its red flower sport were compared in terms of pigment constitution, expression of eight genes related to flavonoid synthesis and sequences of the flavonoid 3',5'hydroxylase (F3'5'H) gene, which is a key gene that synthesizes the pigments of the delphinidin series. According to the HPLC analysis, 'Oomurasaki' contained anthocyanidins from both the cyanidin and delphinidin series, whereas the red flower sport contained only pigments from the cyanidin series. Realtime PCR analysis showed that F3'5'H transcript levels in the red flower sport were 0.14fold those of 'Oomurasaki'. Compared with the amino acid sequences of F3'5'H1A of 'Oomurasaki', both F3'5'H1B and F3'5'H1C sequences of the red flower sport were changed by only one amino acid. These results suggested that low transcript levels of the F3'5'H gene caused no accumulation of delphinidin series pigments in flower colour change between 'Oomurasaki' and its red flower sport.

Original languageEnglish
Pages (from-to)558-562
Number of pages5
JournalPlant Breeding
Volume129
Issue number5
DOIs
Publication statusPublished - Oct 2010

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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