Pigment-dispersing factor affects nocturnal activity rhythms, photic entrainment, and the free-running period of the circadian clock in the cricket gryllus bimaculatus

Ehab Hassaneen, Alaa El-Din Sallam, Ahmad Abo-Ghalia, Yoshiyuki Moriyama, Svetlana G. Karpova, Salah Abdelsalam, Ayami Matsushima, Yasuyuki Shimohigashi, Kenji Tomioka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Pigment-dispersing factor (PDF) is a neuropeptide widely distributed in insect brains and plays important roles in the circadian system. In this study, we used RNA interference to study the role of the pigment-dispersing factor (pdf) gene in regulating circadian locomotor rhythms in the cricket, Gryllus bimaculatus. Injections of pdf double-stranded RNA (dspdf) effectively knocked down the pdf mRNA and PDF peptide levels. The treated crickets maintained the rhythm both under light-dark cycles (LD) and constant darkness (DD). However, they showed rhythms with reduced nocturnal activity with prominent peaks at lights-on and lights-off. Entrainability of dspdf-injected crickets was higher than control crickets as they required fewer cycles to resynchronize to the LD cycles shifted by 6 h. The free-running periods of the dspdf-injected crickets were shorter than those of control crickets in DD. These results suggest that PDF is not essential for the rhythm generation but involved in control of the nocturnality, photic entrainment, and fine tuning of the free-running period of the circadian clock.

Original languageEnglish
Pages (from-to)3-13
Number of pages11
JournalJournal of Biological Rhythms
Volume26
Issue number1
DOIs
Publication statusPublished - Feb 1 2011

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Gryllidae
Circadian Clocks
Photoperiod
Light
Double-Stranded RNA
Darkness
Circadian Rhythm
RNA Interference
Neuropeptides
Insects
Messenger RNA
Peptides
Injections
Brain
Genes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Pigment-dispersing factor affects nocturnal activity rhythms, photic entrainment, and the free-running period of the circadian clock in the cricket gryllus bimaculatus. / Hassaneen, Ehab; El-Din Sallam, Alaa; Abo-Ghalia, Ahmad; Moriyama, Yoshiyuki; Karpova, Svetlana G.; Abdelsalam, Salah; Matsushima, Ayami; Shimohigashi, Yasuyuki; Tomioka, Kenji.

In: Journal of Biological Rhythms, Vol. 26, No. 1, 01.02.2011, p. 3-13.

Research output: Contribution to journalArticle

Hassaneen, Ehab ; El-Din Sallam, Alaa ; Abo-Ghalia, Ahmad ; Moriyama, Yoshiyuki ; Karpova, Svetlana G. ; Abdelsalam, Salah ; Matsushima, Ayami ; Shimohigashi, Yasuyuki ; Tomioka, Kenji. / Pigment-dispersing factor affects nocturnal activity rhythms, photic entrainment, and the free-running period of the circadian clock in the cricket gryllus bimaculatus. In: Journal of Biological Rhythms. 2011 ; Vol. 26, No. 1. pp. 3-13.
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