PirB regulates asymmetries in hippocampal circuitry

Hikari Ukai, Aiko Kawahara, Keiko Hirayama, Matthew Julian Case, Shotaro Aino, Masahiro Miyabe, Ken Wakita, Ryohei Oogi, Michiyo Kasayuki, Shihomi Kawashima, Shunichi Sugimoto, Kanako Chikamatsu, Noritaka Nitta, Tsuneyuki Koga, Ryuichi Shigemoto, Toshiyuki Takai, Isao Ito

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Left-right asymmetry is a fundamental feature of higher-order brain structure; however, the molecular basis of brain asymmetry remains unclear. We recently identified structural and functional asymmetries in mouse hippocampal circuitry that result from the asymmetrical distribution of two distinct populations of pyramidal cell synapses that differ in the density of the NMDA receptor subunit GluRε2 (also known as NR2B, GRIN2B or GluN2B). By examining the synaptic distribution of ε2 subunits, we previously found that β2-microglobulin-deficient mice, which lack cell surface expression of the vast majority of major histocompatibility complex class I (MHCI) proteins, do not exhibit circuit asymmetry. In the present study, we conducted electrophysiological and anatomical analyses on the hippocampal circuitry of mice with a knockout of the paired immunoglobulin-like receptor B (PirB), an MHCI receptor. As in β2-microglobulin-deficient mice, the PirB-deficient hippocampus lacked circuit asymmetries. This finding that MHCI loss-of-function mice and PirB knockout mice have identical phenotypes suggests that MHCI signals that produce hippocampal asymmetries are transduced through PirB. Our results provide evidence for a critical role of the MHCI/PirB signaling system in the generation of asymmetries in hippocampal circuitry.

Original languageEnglish
Article numbere0179377
JournalPloS one
Volume12
Issue number6
DOIs
Publication statusPublished - Jun 1 2017

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Major Histocompatibility Complex
immunoglobulins
Immunoglobulins
major histocompatibility complex
receptors
Brain
mice
Networks (circuits)
N-Methyl-D-Aspartate Receptors
Cells
Pyramidal Cells
Knockout Mice
brain
Synapses
Hippocampus
synapse
hippocampus
Proteins
Phenotype
cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ukai, H., Kawahara, A., Hirayama, K., Case, M. J., Aino, S., Miyabe, M., ... Ito, I. (2017). PirB regulates asymmetries in hippocampal circuitry. PloS one, 12(6), [e0179377]. https://doi.org/10.1371/journal.pone.0179377

PirB regulates asymmetries in hippocampal circuitry. / Ukai, Hikari; Kawahara, Aiko; Hirayama, Keiko; Case, Matthew Julian; Aino, Shotaro; Miyabe, Masahiro; Wakita, Ken; Oogi, Ryohei; Kasayuki, Michiyo; Kawashima, Shihomi; Sugimoto, Shunichi; Chikamatsu, Kanako; Nitta, Noritaka; Koga, Tsuneyuki; Shigemoto, Ryuichi; Takai, Toshiyuki; Ito, Isao.

In: PloS one, Vol. 12, No. 6, e0179377, 01.06.2017.

Research output: Contribution to journalArticle

Ukai, H, Kawahara, A, Hirayama, K, Case, MJ, Aino, S, Miyabe, M, Wakita, K, Oogi, R, Kasayuki, M, Kawashima, S, Sugimoto, S, Chikamatsu, K, Nitta, N, Koga, T, Shigemoto, R, Takai, T & Ito, I 2017, 'PirB regulates asymmetries in hippocampal circuitry', PloS one, vol. 12, no. 6, e0179377. https://doi.org/10.1371/journal.pone.0179377
Ukai H, Kawahara A, Hirayama K, Case MJ, Aino S, Miyabe M et al. PirB regulates asymmetries in hippocampal circuitry. PloS one. 2017 Jun 1;12(6). e0179377. https://doi.org/10.1371/journal.pone.0179377
Ukai, Hikari ; Kawahara, Aiko ; Hirayama, Keiko ; Case, Matthew Julian ; Aino, Shotaro ; Miyabe, Masahiro ; Wakita, Ken ; Oogi, Ryohei ; Kasayuki, Michiyo ; Kawashima, Shihomi ; Sugimoto, Shunichi ; Chikamatsu, Kanako ; Nitta, Noritaka ; Koga, Tsuneyuki ; Shigemoto, Ryuichi ; Takai, Toshiyuki ; Ito, Isao. / PirB regulates asymmetries in hippocampal circuitry. In: PloS one. 2017 ; Vol. 12, No. 6.
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