Pituitary adenylate cyclase activating peptide regulates neurally mediated airway responses

M. Shigyo, H. Aizawa, H. Inoue, Koichiro Matsumoto, S. Takata, N. Hara

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To clarify the protective effects of pituitary adenylate cyclase activating peptide (PACAP) on airway narrowing, we examined the effects of PACAP on smooth muscle contraction and plasma extravasation in guinea-pig airways. Smooth muscle contraction evoked by electrical field stimulation (EFS) or exogenously applied acetylcholine (ACh) or substance P (SP) was measured before and after PACAP in vitro. The effect of PACAP on airway plasma extravasation was also measured in vivo. In trachea, PACAP (10-9-10-7 M) significantly suppressed smooth muscle contraction evoked by EFS without affecting ACh sensitivity, suggesting that PACAP inhibits cholinergic neuroeffector transmission. In the main bronchi, PACAP (10-9-10-8 M) significantly suppressed the contraction evoked by EFS without affecting SP sensitivity in the presence of atropine, suggesting that PACAP inhibits SP release from excitatory nonadrenergic noncholinergic (eNANC) nerves. In animals treated with atropine and propranolol, PACAP attenuated the increase in plasma extravasation induced by electrical vagus stimulation or by SP. These results suggest that pituitary adenylate cyclase activating peptide may play a role in modulation of airway responses through inhibition of cholinergic and noncholinergic mechanisms.

Original languageEnglish
Pages (from-to)64-70
Number of pages7
JournalEuropean Respiratory Journal
Volume12
Issue number1
DOIs
Publication statusPublished - Jul 1 1998

Fingerprint

Adenylyl Cyclases
Peptides
Substance P
Electric Stimulation
Muscle Contraction
Smooth Muscle
Atropine
Cholinergic Agents
Acetylcholine
Bronchi
Trachea
Propranolol
Guinea Pigs

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Pituitary adenylate cyclase activating peptide regulates neurally mediated airway responses. / Shigyo, M.; Aizawa, H.; Inoue, H.; Matsumoto, Koichiro; Takata, S.; Hara, N.

In: European Respiratory Journal, Vol. 12, No. 1, 01.07.1998, p. 64-70.

Research output: Contribution to journalArticle

Shigyo, M. ; Aizawa, H. ; Inoue, H. ; Matsumoto, Koichiro ; Takata, S. ; Hara, N. / Pituitary adenylate cyclase activating peptide regulates neurally mediated airway responses. In: European Respiratory Journal. 1998 ; Vol. 12, No. 1. pp. 64-70.
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