Planar polarization of node cells determines the rotational axis of node cilia

Masakazu Hashimoto, Kyosuke Shinohara, Jianbo Wang, Shingo Ikeuchi, Satoko Yoshiba, Chikara Meno, Shigenori Nonaka, Shinji Takada, Kohei Hatta, Anthony Wynshaw-Boris, Hiroshi Hamada

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Rotational movement of the node cilia generates a leftward fluid flow in the mouse embryo because the cilia are posteriorly tilted. However, it is not known how anterior-posterior information is translated into the posterior tilt of the node cilia. Here, we show that the basal body of node cilia is initially positioned centrally but then gradually shifts toward the posterior side of the node cells. Positioning of the basal body and unidirectional flow were found to be impaired in compound mutant mice lacking Dvl genes. Whereas the basal body was normally positioned in the node cells of Wnt3a-/- embryos, inhibition of Rac1, a component of the noncanonical Wnt signalling pathway, impaired the polarized localization of the basal body in wild-type embryos. Dvl2 and Dvl3 proteins were found to be localized to the apical side of the node cells, and their location was polarized to the posterior side of the cells before the posterior positioning of the basal body. These results suggest that posterior positioning of the basal body, which provides the posterior tilt to node cilia, is determined by planar polarization mediated by noncanonical Wnt signalling.

Original languageEnglish
Pages (from-to)170-176
Number of pages7
JournalNature Cell Biology
Volume12
Issue number2
DOIs
Publication statusPublished - Feb 1 2010

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Basal Bodies
Cilia
Embryonic Structures
Wnt Signaling Pathway
Genes

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Hashimoto, M., Shinohara, K., Wang, J., Ikeuchi, S., Yoshiba, S., Meno, C., ... Hamada, H. (2010). Planar polarization of node cells determines the rotational axis of node cilia. Nature Cell Biology, 12(2), 170-176. https://doi.org/10.1038/ncb2020

Planar polarization of node cells determines the rotational axis of node cilia. / Hashimoto, Masakazu; Shinohara, Kyosuke; Wang, Jianbo; Ikeuchi, Shingo; Yoshiba, Satoko; Meno, Chikara; Nonaka, Shigenori; Takada, Shinji; Hatta, Kohei; Wynshaw-Boris, Anthony; Hamada, Hiroshi.

In: Nature Cell Biology, Vol. 12, No. 2, 01.02.2010, p. 170-176.

Research output: Contribution to journalArticle

Hashimoto, M, Shinohara, K, Wang, J, Ikeuchi, S, Yoshiba, S, Meno, C, Nonaka, S, Takada, S, Hatta, K, Wynshaw-Boris, A & Hamada, H 2010, 'Planar polarization of node cells determines the rotational axis of node cilia', Nature Cell Biology, vol. 12, no. 2, pp. 170-176. https://doi.org/10.1038/ncb2020
Hashimoto M, Shinohara K, Wang J, Ikeuchi S, Yoshiba S, Meno C et al. Planar polarization of node cells determines the rotational axis of node cilia. Nature Cell Biology. 2010 Feb 1;12(2):170-176. https://doi.org/10.1038/ncb2020
Hashimoto, Masakazu ; Shinohara, Kyosuke ; Wang, Jianbo ; Ikeuchi, Shingo ; Yoshiba, Satoko ; Meno, Chikara ; Nonaka, Shigenori ; Takada, Shinji ; Hatta, Kohei ; Wynshaw-Boris, Anthony ; Hamada, Hiroshi. / Planar polarization of node cells determines the rotational axis of node cilia. In: Nature Cell Biology. 2010 ; Vol. 12, No. 2. pp. 170-176.
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