Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule: Towards cell-to-cell communication study

Mitsuhiro Horade, Yoko Mizuta, Noritada Kaji, Tetsuya Higashiyama, Hideyuki Arata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

This paper reports the first observation and quantitative analysis of single cell pollen tube guidance by the signaling molecule realized on the microfluidic device. Design and dimensions of the microfluidic device were optimized for handling individual pollen tube of Torenia Fournieri. Concentration of LURE peptide, the signaling molecule for pollen tube guidance, in the reaction chamber was proved to have appropriate gradient for the guidance. Finally, pollen tube guidance by LURE peptide was successfully observed and analyzed under quantitatively controlled conditions.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1027-1029
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Jan 1 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Horade, M., Mizuta, Y., Kaji, N., Higashiyama, T., & Arata, H. (2012). Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule: Towards cell-to-cell communication study. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1027-1029). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.