Abstract
This paper reports the first observation and quantitative analysis of single cell pollen tube guidance by the signaling molecule realized on the microfluidic device. Design and dimensions of the microfluidic device were optimized for handling individual pollen tube of Torenia Fournieri. Concentration of LURE peptide, the signaling molecule for pollen tube guidance, in the reaction chamber was proved to have appropriate gradient for the guidance. Finally, pollen tube guidance by LURE peptide was successfully observed and analyzed under quantitatively controlled conditions.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1027-1029 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - Jan 1 2012 |
| Externally published | Yes |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: Oct 28 2012 → Nov 1 2012 |
Publication series
| Name | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country | Japan |
| City | Okinawa |
| Period | 10/28/12 → 11/1/12 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule : Towards cell-to-cell communication study. / Horade, Mitsuhiro; Mizuta, Yoko; Kaji, Noritada; Higashiyama, Tetsuya; Arata, Hideyuki.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1027-1029 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
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TY - GEN
T1 - Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule
T2 - Towards cell-to-cell communication study
AU - Horade, Mitsuhiro
AU - Mizuta, Yoko
AU - Kaji, Noritada
AU - Higashiyama, Tetsuya
AU - Arata, Hideyuki
PY - 2012/1/1
Y1 - 2012/1/1
N2 - This paper reports the first observation and quantitative analysis of single cell pollen tube guidance by the signaling molecule realized on the microfluidic device. Design and dimensions of the microfluidic device were optimized for handling individual pollen tube of Torenia Fournieri. Concentration of LURE peptide, the signaling molecule for pollen tube guidance, in the reaction chamber was proved to have appropriate gradient for the guidance. Finally, pollen tube guidance by LURE peptide was successfully observed and analyzed under quantitatively controlled conditions.
AB - This paper reports the first observation and quantitative analysis of single cell pollen tube guidance by the signaling molecule realized on the microfluidic device. Design and dimensions of the microfluidic device were optimized for handling individual pollen tube of Torenia Fournieri. Concentration of LURE peptide, the signaling molecule for pollen tube guidance, in the reaction chamber was proved to have appropriate gradient for the guidance. Finally, pollen tube guidance by LURE peptide was successfully observed and analyzed under quantitatively controlled conditions.
UR - http://www.scopus.com/inward/record.url?scp=84877817347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877817347&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84877817347
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1027
EP - 1029
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
ER -