TY - GEN
T1 - Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
AU - Horade, Mitsuhiro
AU - Mizuta, Yoko
AU - Kaji, Noritada
AU - Higashiyama, Tetsuya
AU - Arata, Hideyuki
PY - 2012
Y1 - 2012
N2 - This paper reports the first observation and quantitative analysis of single cell pollen tube guidance by the signaling molecule realized on the microfluidic device. Design and dimensions of the microfluidic device were optimized for handling individual pollen tube of Torenia Fournieri. Concentration of LURE peptide, the signaling molecule for pollen tube guidance, in the reaction chamber was proved to have appropriate gradient for the guidance. Finally, pollen tube guidance by LURE peptide was successfully observed and analyzed under quantitatively controlled conditions.
AB - This paper reports the first observation and quantitative analysis of single cell pollen tube guidance by the signaling molecule realized on the microfluidic device. Design and dimensions of the microfluidic device were optimized for handling individual pollen tube of Torenia Fournieri. Concentration of LURE peptide, the signaling molecule for pollen tube guidance, in the reaction chamber was proved to have appropriate gradient for the guidance. Finally, pollen tube guidance by LURE peptide was successfully observed and analyzed under quantitatively controlled conditions.
UR - http://www.scopus.com/inward/record.url?scp=84877817347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877817347&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84877817347
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1027
EP - 1029
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
Y2 - 28 October 2012 through 1 November 2012
ER -