Plant protoplast isolation from sterile Acacia mangium seedlings requires β-1,3-glucanase

Toshihiro Ona; Sonoko Tsuda

doi:10.1016/S0176-1617(99)80148-5

Plant protoplast isolation from sterile Acacia mangium seedlings requires β-1,3-glucanase

Toshihiro Ona, Sonoko Tsuda

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The first isolation of plant protoplast using the enzyme β-1,3-glucanase was successfully achieved using sterile Acacia mangium as plant source. Aniline blue reaction and antibodies highly specific to β-1,3-glucans confirmed the induction of β-1,3-glucans in the cell wall by wounding during the mincing treatment which would enhance the accessibility of lytic enzymes. Plant regeneration is expected using this technique.

Original language	English
Pages (from-to)	110-113
Number of pages	4
Journal	Journal of Plant Physiology
Volume	155
Issue number	1
DOIs	https://doi.org/10.1016/S0176-1617(99)80148-5
Publication status	Published - Jan 1 1999
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physiology
Agronomy and Crop Science
Plant Science

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10.1016/S0176-1617(99)80148-5

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abstract = "The first isolation of plant protoplast using the enzyme β-1,3-glucanase was successfully achieved using sterile Acacia mangium as plant source. Aniline blue reaction and antibodies highly specific to β-1,3-glucans confirmed the induction of β-1,3-glucans in the cell wall by wounding during the mincing treatment which would enhance the accessibility of lytic enzymes. Plant regeneration is expected using this technique.",

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AB - The first isolation of plant protoplast using the enzyme β-1,3-glucanase was successfully achieved using sterile Acacia mangium as plant source. Aniline blue reaction and antibodies highly specific to β-1,3-glucans confirmed the induction of β-1,3-glucans in the cell wall by wounding during the mincing treatment which would enhance the accessibility of lytic enzymes. Plant regeneration is expected using this technique.

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Plant protoplast isolation from sterile Acacia mangium seedlings requires β-1,3-glucanase

Abstract

All Science Journal Classification (ASJC) codes

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