The first isolation of plant protoplast using the enzyme β-1,3-glucanase was successfully achieved using sterile Acacia mangium as plant source. Aniline blue reaction and antibodies highly specific to β-1,3-glucans confirmed the induction of β-1,3-glucans in the cell wall by wounding during the mincing treatment which would enhance the accessibility of lytic enzymes. Plant regeneration is expected using this technique.
|Number of pages||4|
|Journal||Journal of Plant Physiology|
|Publication status||Published - Jan 1 1999|
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science