The first isolation of plant protoplast using the enzyme β-1,3-glucanase was successfully achieved using sterile Acacia mangium as plant source. Aniline blue reaction and antibodies highly specific to β-1,3-glucans confirmed the induction of β-1,3-glucans in the cell wall by wounding during the mincing treatment which would enhance the accessibility of lytic enzymes. Plant regeneration is expected using this technique.
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science