Plant protoplast isolation from sterile Acacia mangium seedlings requires β-1,3-glucanase

Toshihiro Ona, Sonoko Tsuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The first isolation of plant protoplast using the enzyme β-1,3-glucanase was successfully achieved using sterile Acacia mangium as plant source. Aniline blue reaction and antibodies highly specific to β-1,3-glucans confirmed the induction of β-1,3-glucans in the cell wall by wounding during the mincing treatment which would enhance the accessibility of lytic enzymes. Plant regeneration is expected using this technique.

Original languageEnglish
Pages (from-to)110-113
Number of pages4
JournalJournal of Plant Physiology
Volume155
Issue number1
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Acacia
Acacia mangium
Protoplasts
Seedlings
protoplasts
Glucans
glucans
seedlings
aniline
Enzymes
enzymes
Cell Wall
Regeneration
cell walls
antibodies
Antibodies
methodology

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Plant protoplast isolation from sterile Acacia mangium seedlings requires β-1,3-glucanase. / Ona, Toshihiro; Tsuda, Sonoko.

In: Journal of Plant Physiology, Vol. 155, No. 1, 01.01.1999, p. 110-113.

Research output: Contribution to journalArticle

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