Plasmacytoid dendritic cells activate lymphoid-specific genetic programs irrespective of their cellular origin

Hirokazu Shigematsu, Boris Reizis, Hiromi Iwasaki, Shinichi Mizuno, Dan Hu, David Traver, Philip Leder, Nobuo Sakaguchi, Koichi Akashi

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

The developmental origin of type I interferon (IFN)-producing plasmacytoid dendritic cells (PDCs) is controversial. In particular, the rearrangement of immunoglobulin heavy chain (IgH) genes in murine PDCs and the expression of pre-T cell receptor α (pTα) gene by human PDCs were proposed as evidence for their "lymphoid" origin. Here we demonstrate that PDCs capable of IFN production develop efficiently from both myeloid- and lymphoid-committed progenitors. Rearranged IgH genes as well as RAG transcripts were found in both myeloid- and lymphoid-derived PDCs. The human pTα transgenic reporter was activated in both myeloid- and lymphoid-derived PDCs at a level comparable to pre-T cells. PDCs were the only cell population that activated murine RAG1 knockin and human pTα transgenic reporters outside the lymphoid lineage. These results highlight a unique developmental program of PDCs that distinguishes them from other cell types including conventional dendritic cells.

Original languageEnglish
Pages (from-to)43-53
Number of pages11
JournalImmunity
Volume21
Issue number1
DOIs
Publication statusPublished - Jul 1 2004

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Dendritic Cells
Immunoglobulin Heavy Chain Genes
T-Cell Receptor Genes
Interferon Type I
Interferons
T-Lymphocytes
Population

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Plasmacytoid dendritic cells activate lymphoid-specific genetic programs irrespective of their cellular origin. / Shigematsu, Hirokazu; Reizis, Boris; Iwasaki, Hiromi; Mizuno, Shinichi; Hu, Dan; Traver, David; Leder, Philip; Sakaguchi, Nobuo; Akashi, Koichi.

In: Immunity, Vol. 21, No. 1, 01.07.2004, p. 43-53.

Research output: Contribution to journalArticle

Shigematsu, H, Reizis, B, Iwasaki, H, Mizuno, S, Hu, D, Traver, D, Leder, P, Sakaguchi, N & Akashi, K 2004, 'Plasmacytoid dendritic cells activate lymphoid-specific genetic programs irrespective of their cellular origin', Immunity, vol. 21, no. 1, pp. 43-53. https://doi.org/10.1016/j.immuni.2004.06.011
Shigematsu, Hirokazu ; Reizis, Boris ; Iwasaki, Hiromi ; Mizuno, Shinichi ; Hu, Dan ; Traver, David ; Leder, Philip ; Sakaguchi, Nobuo ; Akashi, Koichi. / Plasmacytoid dendritic cells activate lymphoid-specific genetic programs irrespective of their cellular origin. In: Immunity. 2004 ; Vol. 21, No. 1. pp. 43-53.
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