Abstract
After treatment with mitomycin C and other antibacterial agents, a translucent, smooth-colony-forming mycobacterium, isolated from sputum and designated as M. intracellulare strain 103, gave rise to variants forming opaque colonies. These opaque variants were more sensitive streptomycin, kanamycin, viomycin, and rifampin than were the wild-type translucent variants. Plasmid deoxyribonucleic acids taken from translucent strain cells and from cells of certain opaque variants were analyzed by agarose gel electrophoresis. Two plasmids of molecular weights of approximately 2 X 106 and 50 X 106, respectively, were found in the wild-type translucent cells; one of them, the 2 X 106-molecular-weight plasmid, was always missing from deoxyribonucleic acids of the opaque variant cells. The results suggested that translucent colonial appearance and antibiotic resistance of the strain are plasmid-determined functions.
| Original language | English |
|---|---|
| Pages (from-to) | 656-659 |
| Number of pages | 4 |
| Journal | Journal of Bacteriology |
| Volume | 146 |
| Issue number | 2 |
| Publication status | Published - 1981 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology
- Immunology
Cite this
Plasmid deoxyribonucleic acid and translucent-to-opaque variation in Mycobacterium intracellulare 103. / Mizuguchi, Y.; Fukunaga, M.; Taniguchi, Hatsumi.
In: Journal of Bacteriology, Vol. 146, No. 2, 1981, p. 656-659.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasmid deoxyribonucleic acid and translucent-to-opaque variation in Mycobacterium intracellulare 103
AU - Mizuguchi, Y.
AU - Fukunaga, M.
AU - Taniguchi, Hatsumi
PY - 1981
Y1 - 1981
N2 - After treatment with mitomycin C and other antibacterial agents, a translucent, smooth-colony-forming mycobacterium, isolated from sputum and designated as M. intracellulare strain 103, gave rise to variants forming opaque colonies. These opaque variants were more sensitive streptomycin, kanamycin, viomycin, and rifampin than were the wild-type translucent variants. Plasmid deoxyribonucleic acids taken from translucent strain cells and from cells of certain opaque variants were analyzed by agarose gel electrophoresis. Two plasmids of molecular weights of approximately 2 X 106 and 50 X 106, respectively, were found in the wild-type translucent cells; one of them, the 2 X 106-molecular-weight plasmid, was always missing from deoxyribonucleic acids of the opaque variant cells. The results suggested that translucent colonial appearance and antibiotic resistance of the strain are plasmid-determined functions.
AB - After treatment with mitomycin C and other antibacterial agents, a translucent, smooth-colony-forming mycobacterium, isolated from sputum and designated as M. intracellulare strain 103, gave rise to variants forming opaque colonies. These opaque variants were more sensitive streptomycin, kanamycin, viomycin, and rifampin than were the wild-type translucent variants. Plasmid deoxyribonucleic acids taken from translucent strain cells and from cells of certain opaque variants were analyzed by agarose gel electrophoresis. Two plasmids of molecular weights of approximately 2 X 106 and 50 X 106, respectively, were found in the wild-type translucent cells; one of them, the 2 X 106-molecular-weight plasmid, was always missing from deoxyribonucleic acids of the opaque variant cells. The results suggested that translucent colonial appearance and antibiotic resistance of the strain are plasmid-determined functions.
UR - http://www.scopus.com/inward/record.url?scp=0019827459&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019827459&partnerID=8YFLogxK
M3 - Article
C2 - 7217014
AN - SCOPUS:0019827459
VL - 146
SP - 656
EP - 659
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 2
ER -