Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet

Kosuke Ino, Tamayo Kawasumi, Akira Ito, Hiroyuki Honda

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Modification of cellular functions by over-expression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA/MCL) and magnetic force. The transfection efficiencies of the magnetofection technique by pDNA/MCL in fibroblasts and keratinocytes using reporter genes were 36- and 10-fold higher, respectively, than those of a lipofection technique by cationic liposomes. Moreover, in vitro construction of three-dimensional (3D) tissues is an important challenge. We recently proposed a novel technique termed "magnetic force-based tissue engineering" (Mag-TE) to produce 3D tissues. Since the fibroblasts after magnetofection incorporated both magnetite nanoparticles and pDNA, we investigated whether multilayered heterotypic cell sheets expressing transgene could be fabricated by Mag-TE. First, the fibroblasts were seeded onto an ultra-low attachment culture plate. When a magnet was placed under the plate, the cells accumulated at the bottom of the culture plate. After 24 h of culture, the transgene-expressing cells formed a multilayered cell sheet-like structure. These results indicated that MCLs are a potent biomanipulation tool for both gene transfer and 3D tissue construction, suggesting that these techniques are useful for tissue engineering.

Original languageEnglish
Pages (from-to)168-176
Number of pages9
JournalBiotechnology and Bioengineering
Volume100
Issue number1
DOIs
Publication statusPublished - May 1 2008

Fingerprint

Ferrosoferric Oxide
Liposomes
Magnetite
Transfection
Tissue Engineering
Plasmids
DNA
Tissue engineering
Genes
Fibroblasts
Tissue
Transgenes
Magnetite Nanoparticles
Gene transfer
Magnetite nanoparticles
Magnets
Keratinocytes
Reporter Genes
Cell culture
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet. / Ino, Kosuke; Kawasumi, Tamayo; Ito, Akira; Honda, Hiroyuki.

In: Biotechnology and Bioengineering, Vol. 100, No. 1, 01.05.2008, p. 168-176.

Research output: Contribution to journalArticle

Ino, Kosuke ; Kawasumi, Tamayo ; Ito, Akira ; Honda, Hiroyuki. / Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet. In: Biotechnology and Bioengineering. 2008 ; Vol. 100, No. 1. pp. 168-176.
@article{8a5425d153284b19be16b118aafa8eb7,
title = "Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet",
abstract = "Modification of cellular functions by over-expression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA/MCL) and magnetic force. The transfection efficiencies of the magnetofection technique by pDNA/MCL in fibroblasts and keratinocytes using reporter genes were 36- and 10-fold higher, respectively, than those of a lipofection technique by cationic liposomes. Moreover, in vitro construction of three-dimensional (3D) tissues is an important challenge. We recently proposed a novel technique termed {"}magnetic force-based tissue engineering{"} (Mag-TE) to produce 3D tissues. Since the fibroblasts after magnetofection incorporated both magnetite nanoparticles and pDNA, we investigated whether multilayered heterotypic cell sheets expressing transgene could be fabricated by Mag-TE. First, the fibroblasts were seeded onto an ultra-low attachment culture plate. When a magnet was placed under the plate, the cells accumulated at the bottom of the culture plate. After 24 h of culture, the transgene-expressing cells formed a multilayered cell sheet-like structure. These results indicated that MCLs are a potent biomanipulation tool for both gene transfer and 3D tissue construction, suggesting that these techniques are useful for tissue engineering.",
author = "Kosuke Ino and Tamayo Kawasumi and Akira Ito and Hiroyuki Honda",
year = "2008",
month = "5",
day = "1",
doi = "10.1002/bit.21738",
language = "English",
volume = "100",
pages = "168--176",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Plasmid DNA transfection using magnetite cationic liposomes for construction of multilayered gene-engineered cell sheet

AU - Ino, Kosuke

AU - Kawasumi, Tamayo

AU - Ito, Akira

AU - Honda, Hiroyuki

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Modification of cellular functions by over-expression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA/MCL) and magnetic force. The transfection efficiencies of the magnetofection technique by pDNA/MCL in fibroblasts and keratinocytes using reporter genes were 36- and 10-fold higher, respectively, than those of a lipofection technique by cationic liposomes. Moreover, in vitro construction of three-dimensional (3D) tissues is an important challenge. We recently proposed a novel technique termed "magnetic force-based tissue engineering" (Mag-TE) to produce 3D tissues. Since the fibroblasts after magnetofection incorporated both magnetite nanoparticles and pDNA, we investigated whether multilayered heterotypic cell sheets expressing transgene could be fabricated by Mag-TE. First, the fibroblasts were seeded onto an ultra-low attachment culture plate. When a magnet was placed under the plate, the cells accumulated at the bottom of the culture plate. After 24 h of culture, the transgene-expressing cells formed a multilayered cell sheet-like structure. These results indicated that MCLs are a potent biomanipulation tool for both gene transfer and 3D tissue construction, suggesting that these techniques are useful for tissue engineering.

AB - Modification of cellular functions by over-expression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA/MCL) and magnetic force. The transfection efficiencies of the magnetofection technique by pDNA/MCL in fibroblasts and keratinocytes using reporter genes were 36- and 10-fold higher, respectively, than those of a lipofection technique by cationic liposomes. Moreover, in vitro construction of three-dimensional (3D) tissues is an important challenge. We recently proposed a novel technique termed "magnetic force-based tissue engineering" (Mag-TE) to produce 3D tissues. Since the fibroblasts after magnetofection incorporated both magnetite nanoparticles and pDNA, we investigated whether multilayered heterotypic cell sheets expressing transgene could be fabricated by Mag-TE. First, the fibroblasts were seeded onto an ultra-low attachment culture plate. When a magnet was placed under the plate, the cells accumulated at the bottom of the culture plate. After 24 h of culture, the transgene-expressing cells formed a multilayered cell sheet-like structure. These results indicated that MCLs are a potent biomanipulation tool for both gene transfer and 3D tissue construction, suggesting that these techniques are useful for tissue engineering.

UR - http://www.scopus.com/inward/record.url?scp=42549140629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42549140629&partnerID=8YFLogxK

U2 - 10.1002/bit.21738

DO - 10.1002/bit.21738

M3 - Article

C2 - 18078300

AN - SCOPUS:42549140629

VL - 100

SP - 168

EP - 176

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 1

ER -