Plasmin induces endothelium-dependent nitric oxide-mediated relaxation in the porcine coronary artery

Tetsuhiro Fujiyoshi, Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Shosuke Takahashi, Hideo Kanaide

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

OBJECTIVE - Plasmin is a key enzyme in fibrinolysis. We attempted to determine the possible role of plasmin in the regulation of vascular tone, while also investigating the mechanism of plasmin-induced vasorelaxation. METHODS AND RESULTS - In porcine coronary artery, plasmin induced an endothelium-dependent relaxation. This relaxing effect was mostly abolished by a proteinase inhibitor, a plasmin inhibitor, or a nitric oxide (NO) synthase inhibitor. The preceding stimulation with plasmin significantly inhibited the subsequent relaxation induced by thrombin but not that induced by proteinase-activated receptor-1-activating peptide. The relaxation induced by trypsin and substance P remained unaffected by the preceding plasmin stimulation. The pretreatment with plasmin, thrombin, or trypsin significantly attenuated the plasmin-induced relaxation. In porcine coronary artery endothelial cells (PCAECs) and human umbilical vein endothelial cells (HUVECs), plasmin induced a transient elevation in the cytosolic Ca concentrations ([Ca]i). The preceding stimulation with plasmin inhibited the subsequent [Ca]i elevation induced by thrombin but not that induced by trypsin. In PCAECs, plasmin concentration-dependently induced NO production. CONCLUSIONS - The present study demonstrated, for the first time, that plasmin induced an endothelium-dependent NO-mediated relaxation in the porcine coronary artery, while also showing plasmin to specifically inactivate the thrombin receptor.

Original languageEnglish
Pages (from-to)949-954
Number of pages6
JournalArteriosclerosis, thrombosis, and vascular biology
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 2007

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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