Platelet-activating factor acetylhydrolase gene polymorphism and its activity in Japanese patients with multiple sclerosis

Manabu Osoegawa, Masaaki Niino, Hirofumi Ochi, Seiji Kikuchi, Hiroyuki Murai, Toshiyuki Fukazawa, Motozumi Minohara, Kunio Tashiro, Jun Ichi Kira

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14 Citations (Scopus)

Abstract

We evaluated the association of the plasma platelet-activating factor acetylhydrolase (PAF-AH) gene polymorphism (G994→T) and PAF-AH activity with susceptibility and severity of multiple sclerosis (MS) in Japanese. DNA was collected from 216 patients with clinically definite MS (65 opticospinal MS (OS-MS) and 151 conventional MS (C-MS)) and from 213 healthy controls. The missense mutation G994→T that disrupts the PAF-AH activity was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). No statistically significant difference in the frequency of genotypes and alleles of the plasma PAF-AH polymorphism was observed among OS-MS patients, C-MS patients and healthy controls. However, the missense mutation tended to be associated with the severity of OS-MS, especially in females (GT/TT genotypes; 51.7% in female rapidly progressive OS-MS vs. 26.6% in female controls, p=0.0870). Moreover, PAF-AH activities were significantly lower in MS than in controls, irrespective of clinical subtypes, among those carrying the identical polymorphism in terms of nucleotide position 994 of the PAF-AH gene. These findings suggest that the PAF-AH gene missense mutation has no relation to either susceptibility or severity of C-MS, yet its activity is down-regulated, and that the mutation has no relation with susceptibility of OS-MS, yet it may confer the severity of female OS-MS.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalJournal of Neuroimmunology
Volume150
Issue number1-2
DOIs
Publication statusPublished - May 2004

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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    Osoegawa, M., Niino, M., Ochi, H., Kikuchi, S., Murai, H., Fukazawa, T., Minohara, M., Tashiro, K., & Kira, J. I. (2004). Platelet-activating factor acetylhydrolase gene polymorphism and its activity in Japanese patients with multiple sclerosis. Journal of Neuroimmunology, 150(1-2), 150-156. https://doi.org/10.1016/j.jneuroim.2004.01.008