Platelet stimulation for prostacyclin production in aortic endothelial cell cultures: Alteration in diabetes mellitus

Toyoshi Inoguchi, F. Umeda, M. Kunisaki, H. Ishii, T. Yamauchi, H. Nawata

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    We evaluated the effect of platelets on prostacyclin (PGI2) production in bovine aortic endothelial cell cultures. Human platelet extract significantly stimulated PGI2 production by cultured aortic endothelial cells in a time- and dose-dependent manner, suggesting that platelets contain PGI2-stimulatory activity (PSA). Supernatant fluid separated from platelets activated by collagen also exhibited PSA. The factor(s) causing the PSA of platelets was non-dialysable and heat-stable (56°C for 30 min or 100°C for 3 min), was completely inhibited by trypsin pretreatment, and exhibited an affinity to heparin agarose. Furthermore, gel filtration chromatography showed that the factor(s) responsible for the platelet PSA was eluted at three different peaks with approximate molecular weights of 50,000, 25,000 and 11,000. The PSA of platelet extract from patients with non-insulin-dependent diabetes mellitus (NIDDM) (n = 10) was compared to that from age-matched control subjects (n = 10). Platelet extract from patients with NIDDM stimulated cultured aortic endothelial cells to produce greater amounts of PGI2 than did that from control subjects. These data suggest that the increased PSA of platelets isolated from diabetic patients may contribute to the abnormal interaction between platelets and the vascular wall in diabetic patients.

    Original languageEnglish
    Pages (from-to)539-544
    Number of pages6
    JournalHormone and Metabolic Research
    Volume23
    Issue number11
    DOIs
    Publication statusPublished - Jan 1 1991

    Fingerprint

    Endothelial cells
    Epoprostenol
    Medical problems
    Platelets
    Cell culture
    Diabetes Mellitus
    Blood Platelets
    Endothelial Cells
    Cell Culture Techniques
    Type 2 Diabetes Mellitus
    Chromatography
    Trypsin
    Gel Chromatography
    Blood Vessels
    Collagen
    Hot Temperature
    Molecular Weight
    Gels
    Molecular weight
    Fluids

    All Science Journal Classification (ASJC) codes

    • Endocrinology, Diabetes and Metabolism
    • Biochemistry
    • Endocrinology
    • Clinical Biochemistry
    • Biochemistry, medical

    Cite this

    Platelet stimulation for prostacyclin production in aortic endothelial cell cultures : Alteration in diabetes mellitus. / Inoguchi, Toyoshi; Umeda, F.; Kunisaki, M.; Ishii, H.; Yamauchi, T.; Nawata, H.

    In: Hormone and Metabolic Research, Vol. 23, No. 11, 01.01.1991, p. 539-544.

    Research output: Contribution to journalArticle

    Inoguchi, Toyoshi ; Umeda, F. ; Kunisaki, M. ; Ishii, H. ; Yamauchi, T. ; Nawata, H. / Platelet stimulation for prostacyclin production in aortic endothelial cell cultures : Alteration in diabetes mellitus. In: Hormone and Metabolic Research. 1991 ; Vol. 23, No. 11. pp. 539-544.
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