PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases

Hiromu Ikeda; Yoshihiro Yayama; Akito Hata; Jumpei Kamimoto; Tatsuhiro Yamamoto; Takeshi Mori; Yoshiki Katayama

doi:10.2116/analsci.30.631

PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases

Hiromu Ikeda, Yoshihiro Yayama, Akito Hata, Jumpei Kamimoto, Tatsuhiro Yamamoto, Takeshi Mori, Yoshiki Katayama

Materials Science and Engineering, School of Engineering

Research output: Contribution to journal › Article

Abstract

Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

Original language	English
Pages (from-to)	631-635
Number of pages	5
Journal	analytical sciences
Volume	30
Issue number	6
DOIs	https://doi.org/10.2116/analsci.30.631
Publication status	Published - 2014

Fingerprint

All Science Journal Classification (ASJC) codes

Analytical Chemistry

Cite this

Ikeda, H., Yayama, Y., Hata, A., Kamimoto, J., Yamamoto, T., Mori, T., & Katayama, Y. (2014). PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases. analytical sciences, 30(6), 631-635. https://doi.org/10.2116/analsci.30.631

@article{30fa95216b084c5a8ab037393d9edf6b,

title = "PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases",

abstract = "Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.",

author = "Hiromu Ikeda and Yoshihiro Yayama and Akito Hata and Jumpei Kamimoto and Tatsuhiro Yamamoto and Takeshi Mori and Yoshiki Katayama",

year = "2014",

doi = "10.2116/analsci.30.631",

language = "English",

volume = "30",

pages = "631--635",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "The Japan Society for Analytical Chemistry",

number = "6",

}

TY - JOUR

T1 - PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases

AU - Ikeda, Hiromu

AU - Yayama, Yoshihiro

AU - Hata, Akito

AU - Kamimoto, Jumpei

AU - Yamamoto, Tatsuhiro

AU - Mori, Takeshi

AU - Katayama, Yoshiki

PY - 2014

Y1 - 2014

N2 - Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

AB - Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

UR - http://www.scopus.com/inward/record.url?scp=84902132329&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902132329&partnerID=8YFLogxK

U2 - 10.2116/analsci.30.631

DO - 10.2116/analsci.30.631

M3 - Article

C2 - 24919666

AN - SCOPUS:84902132329

VL - 30

SP - 631

EP - 635

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 6

ER -

Access to Document

10.2116/analsci.30.631