PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases

Hiromu Ikeda, Yoshihiro Yayama, Akito Hata, Jumpei Kamimoto, Tatsuhiro Yamamoto, Takeshi Mori, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

    Original languageEnglish
    Pages (from-to)631-635
    Number of pages5
    Journalanalytical sciences
    Volume30
    Issue number6
    DOIs
    Publication statusPublished - 2014

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry

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