Podoplanin expression in the cyst wall correlates with the progression of intraductal papillary mucinous neoplasm.

Koji Shindo, Shinichi Aishima, Kenoki Ohuchida, Minoru Fujino, Yusuke Mizuuchi, Masami Hattori, Takao Ohtsuka, Shoji Tokunaga, Kazuhiro Mizumoto, Masao Tanaka, Yoshinao Oda

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Abstract

A thickened, enhanced cyst wall on imaging examinations is one of the "worrisome features" described in the consensus guidelines for management of intraductal papillary mucinous neoplasm of the pancreas (IPMN). Podoplanin (PDPN) expression by cancer-associated fibroblasts is known to be an indicator of poor prognosis in some types of cancer. We performed immunohistochemical staining for alpha-smooth muscle actin (α-SMA) in IPMN lesions and determined the pathological wall thickness by measuring the thinnest and thickest α-SMA-positive parts of the wall of the largest cyst in each case, and the mean of these two values was recorded as the wall thickness. The thickness of the pathological wall increased with progression from IPMN with low-grade dysplasia to IPMN with an invasive carcinoma. The pathological wall was thicker in IPMN with main duct involvement, nongastric-type IPMN, and IPMN with mural nodules. We also stained for PDPN and assessed the thickness of cyst wall staining as for α-SMA. The thickness of the PDPN-positive cyst wall varied in a pattern similar to the thickness of the α-SMA-positive pathological cyst wall. PDPN-positive stromal fibroblasts in the invasive component of IPMN-IC were evaluated as a ratio to α-SMA-positive fibroblasts. A high ratio (>50 %) of PDPN-positive stromal fibroblasts was a predictor of poor outcome. PDPN expression in the cyst wall correlates with the progression of IPMN. PDPN may be a significant prognostic marker of IPMN-IC.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalVirchows Archiv : an international journal of pathology
Volume465
Issue number3
DOIs
Publication statusPublished - Sep 2014

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Pancreatic Neoplasms
Cysts
Neoplasms
Fibroblasts
Staining and Labeling
Smooth Muscle
Actins
Guidelines
Carcinoma

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Podoplanin expression in the cyst wall correlates with the progression of intraductal papillary mucinous neoplasm. / Shindo, Koji; Aishima, Shinichi; Ohuchida, Kenoki; Fujino, Minoru; Mizuuchi, Yusuke; Hattori, Masami; Ohtsuka, Takao; Tokunaga, Shoji; Mizumoto, Kazuhiro; Tanaka, Masao; Oda, Yoshinao.

In: Virchows Archiv : an international journal of pathology, Vol. 465, No. 3, 09.2014, p. 265-273.

Research output: Contribution to journalArticle

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