Point mutation assay by affinity capillary electrophoresis using poly(ethylene glycol)-oligodeoxyribonucleotide block copolymers.

Ayumi Kimura, Tohru Takarada, Naoki Kanayama, Hideaki Shibata, Mizuo Maeda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An affinity capillary electrophoresis was developed for detecting a point mutation of single-stranded DNA (ssDNA). Poly(ethylene glycol)-oligodeoxyribonucleo-tide block copolymers (PEG-b-ODN) were prepared to use as a novel affinity ligand. Optimization of the analytical conditions, such as the salt concentration of running buffer, the capillary temperature and the base number of the copolymer, gave two distinct peaks for wild-type (WT) and point-mutated ssDNA (MT) on the electropherogram, allowing for the facile discrimination of the single-base difference.

Original languageEnglish
Pages (from-to)337-338
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007
Externally publishedYes

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Ethylene Glycol
Oligodeoxyribonucleotides
Single-Stranded DNA
Capillary Electrophoresis
Point Mutation
Buffers
Salts
Ligands
Temperature

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Point mutation assay by affinity capillary electrophoresis using poly(ethylene glycol)-oligodeoxyribonucleotide block copolymers. / Kimura, Ayumi; Takarada, Tohru; Kanayama, Naoki; Shibata, Hideaki; Maeda, Mizuo.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 337-338.

Research output: Contribution to journalArticle

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