TY - JOUR
T1 - Pol B, a family B DNA polymerase, in Thermococcus kodakarensis is important for DNA repair, but not DNA replication
AU - Kushida, Takashi
AU - Narumi, Issay
AU - Ishino, Sonoko
AU - Ishino, Yoshizumi
AU - Fujiwara, Shinsuke
AU - Imanaka, Tadayuki
AU - Higashibata, Hiroki
N1 - Funding Information:
This study was supported in part by the Inoue Enryo Memorial Foundation for Promoting Sciences.
Publisher Copyright:
© 2019, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Thermococcus kodakarensis possesses two DNA polymerases, Pol B and Pol D. We generated a T. kodakarensis strain (DPB1) in which polB was completely deleted and a derivative of DPB1 in which polB was overexpressed; neither of the generated strains exhibited any growth delay, indicating that the lack or overexpression of Pol B in T. kodakarensis did not affect cell growth. We also found that DPB1 showed higher sensitivity to four DNA-damaging agents (ultraviolet C irradiation, γ-ray irradiation, methyl methanesulfonate, and mitomycin C) than the parental strain. The sensitivity of DPB1 was restored to the level of the parent strain by the introduction of a plasmid harboring polB, suggesting that the DNA damage-sensitive phenotype of DPB1 was due to the loss of polB. Collectively, these results indicate that Pol B is involved in DNA repair, but not DNA replication, which, in turn, implies that Pol D is the sole replicative DNA polymerase in Thermococcus species.
AB - Thermococcus kodakarensis possesses two DNA polymerases, Pol B and Pol D. We generated a T. kodakarensis strain (DPB1) in which polB was completely deleted and a derivative of DPB1 in which polB was overexpressed; neither of the generated strains exhibited any growth delay, indicating that the lack or overexpression of Pol B in T. kodakarensis did not affect cell growth. We also found that DPB1 showed higher sensitivity to four DNA-damaging agents (ultraviolet C irradiation, γ-ray irradiation, methyl methanesulfonate, and mitomycin C) than the parental strain. The sensitivity of DPB1 was restored to the level of the parent strain by the introduction of a plasmid harboring polB, suggesting that the DNA damage-sensitive phenotype of DPB1 was due to the loss of polB. Collectively, these results indicate that Pol B is involved in DNA repair, but not DNA replication, which, in turn, implies that Pol D is the sole replicative DNA polymerase in Thermococcus species.
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U2 - 10.1264/jsme2.ME19075
DO - 10.1264/jsme2.ME19075
M3 - Article
C2 - 31353332
AN - SCOPUS:85072686855
SN - 1342-6311
VL - 34
SP - 316
EP - 326
JO - Microbes and Environments
JF - Microbes and Environments
IS - 3
ER -