Pol B, a family B DNA polymerase, in Thermococcus kodakarensis is important for DNA repair, but not DNA replication

Takashi Kushida, Issay Narumi, Sonoko Ishino, Yoshizumi Ishino, Shinsuke Fujiwara, Tadayuki Imanaka, Hiroki Higashibata

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Thermococcus kodakarensis possesses two DNA polymerases, Pol B and Pol D. We generated a T. kodakarensis strain (DPB1) in which polB was completely deleted and a derivative of DPB1 in which polB was overexpressed; neither of the generated strains exhibited any growth delay, indicating that the lack or overexpression of Pol B in T. kodakarensis did not affect cell growth. We also found that DPB1 showed higher sensitivity to four DNA-damaging agents (ultraviolet C irradiation, γ-ray irradiation, methyl methanesulfonate, and mitomycin C) than the parental strain. The sensitivity of DPB1 was restored to the level of the parent strain by the introduction of a plasmid harboring polB, suggesting that the DNA damage-sensitive phenotype of DPB1 was due to the loss of polB. Collectively, these results indicate that Pol B is involved in DNA repair, but not DNA replication, which, in turn, implies that Pol D is the sole replicative DNA polymerase in Thermococcus species.

Original languageEnglish
Pages (from-to)316-326
Number of pages11
JournalMicrobes and Environments
Volume34
Issue number3
DOIs
Publication statusPublished - Jan 1 2019

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Thermococcus kodakarensis
DNA-directed DNA polymerase
DNA replication
DNA repair
repair
DNA
Thermococcus
irradiation
methyl methanesulfonate
mitomycin
DNA damage
ultraviolet radiation
cell growth
plasmids
plasmid
phenotype
family
damage

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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Pol B, a family B DNA polymerase, in Thermococcus kodakarensis is important for DNA repair, but not DNA replication. / Kushida, Takashi; Narumi, Issay; Ishino, Sonoko; Ishino, Yoshizumi; Fujiwara, Shinsuke; Imanaka, Tadayuki; Higashibata, Hiroki.

In: Microbes and Environments, Vol. 34, No. 3, 01.01.2019, p. 316-326.

Research output: Contribution to journalArticle

Kushida, Takashi ; Narumi, Issay ; Ishino, Sonoko ; Ishino, Yoshizumi ; Fujiwara, Shinsuke ; Imanaka, Tadayuki ; Higashibata, Hiroki. / Pol B, a family B DNA polymerase, in Thermococcus kodakarensis is important for DNA repair, but not DNA replication. In: Microbes and Environments. 2019 ; Vol. 34, No. 3. pp. 316-326.
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