Polarity in archaeal operon transcription in Thermococcus kodakaraensis

Thomas J. Santangelo, L'ubomíra Čuboňová, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, John N. Reeve

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

Original languageEnglish
Pages (from-to)2244-2248
Number of pages5
JournalJournal of bacteriology
Volume190
Issue number6
DOIs
Publication statusPublished - Mar 1 2008

Fingerprint

Thermococcus
Operon
Archaeal Genes
Nonsense Codon
Glycoside Hydrolases
Archaea
Reporter Genes
Genes
Gene Expression

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Santangelo, T. J., Čuboňová, L., Matsumi, R., Atomi, H., Imanaka, T., & Reeve, J. N. (2008). Polarity in archaeal operon transcription in Thermococcus kodakaraensis. Journal of bacteriology, 190(6), 2244-2248. https://doi.org/10.1128/JB.01811-07

Polarity in archaeal operon transcription in Thermococcus kodakaraensis. / Santangelo, Thomas J.; Čuboňová, L'ubomíra; Matsumi, Rie; Atomi, Haruyuki; Imanaka, Tadayuki; Reeve, John N.

In: Journal of bacteriology, Vol. 190, No. 6, 01.03.2008, p. 2244-2248.

Research output: Contribution to journalArticle

Santangelo, TJ, Čuboňová, L, Matsumi, R, Atomi, H, Imanaka, T & Reeve, JN 2008, 'Polarity in archaeal operon transcription in Thermococcus kodakaraensis', Journal of bacteriology, vol. 190, no. 6, pp. 2244-2248. https://doi.org/10.1128/JB.01811-07
Santangelo TJ, Čuboňová L, Matsumi R, Atomi H, Imanaka T, Reeve JN. Polarity in archaeal operon transcription in Thermococcus kodakaraensis. Journal of bacteriology. 2008 Mar 1;190(6):2244-2248. https://doi.org/10.1128/JB.01811-07
Santangelo, Thomas J. ; Čuboňová, L'ubomíra ; Matsumi, Rie ; Atomi, Haruyuki ; Imanaka, Tadayuki ; Reeve, John N. / Polarity in archaeal operon transcription in Thermococcus kodakaraensis. In: Journal of bacteriology. 2008 ; Vol. 190, No. 6. pp. 2244-2248.
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