Polarity in archaeal operon transcription in Thermococcus kodakaraensis

Thomas J. Santangelo, L'ubomíra Čuboňová, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, John N. Reeve

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

Original languageEnglish
Pages (from-to)2244-2248
Number of pages5
JournalJournal of bacteriology
Volume190
Issue number6
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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