Polarized localization and borate-dependent degradation of the Arabidopsis borate transporter BOR1 in tobacco BY-2 cells

Ken Matsuoka, Noboru Yamauchi, Tadashi Gosho, Satoru Asatuma, Kiminori Toyooka, Toru Fujiwara

Research output: Contribution to journalArticle

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Abstract

In Arabidopsis the borate transporter BOR1, which is located in the plasma membrane, is degraded in the presence of excess boron by an endocytosis-mediated mechanism. A similar mechanism was suggested in rice as excess boron decreased rice borate transporter levels, although in this case whether the decrease was dependent on an increase in degradation or a decrease in protein synthesis was not elucidated. To address whether the borate-dependent degradation mechanism is conserved among plant cells, we analyzed the fate of GFP-tagged BOR1 (BOR1-GFP) in transformed tobacco BY-2 cells. Cells expressing BOR1-GFP displayed GFP fluorescence at the plasma membrane, especially at the membrane between two attached cells. The plasma membrane signal was abolished when cells were incubated in medium with a high concentration of borate (3 to 5 mM). This decrease in BOR1-GFP signal was mediated by a specific degradation of the protein after internalization by endocytosis from the plasma membrane. Pharmacological analysis indicated that the decrease in BOR1-GFP largely depends on the increase in degradation rate and that the degradation was mediated by a tyrosine-motif and the actin cytoskeleton. Tyr mutants of BOR1-GFP, which has been shown to inhibit borate-dependent degradation in Arabidopsis root cells, did not show borate-dependent endocytosis in tobacco BY-2 cells. These findings indicate that the borate-dependent degradation machinery of the borate transporter is conserved among plant species.

Original languageEnglish
Article number185.v1
JournalF1000Research
Volume2
DOIs
Publication statusPublished - Sep 13 2013

Fingerprint

Borates
Tobacco
Degradation
Cell membranes
Endocytosis
Cell Membrane
Boron
Arabidopsis BOR1 protein
Plant Cells
Actin Cytoskeleton
Arabidopsis
Proteolysis
Machinery
Tyrosine
Actins
Proteins
Fluorescence
Cells
Pharmacology
Membranes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Polarized localization and borate-dependent degradation of the Arabidopsis borate transporter BOR1 in tobacco BY-2 cells. / Matsuoka, Ken; Yamauchi, Noboru; Gosho, Tadashi; Asatuma, Satoru; Toyooka, Kiminori; Fujiwara, Toru.

In: F1000Research, Vol. 2, 185.v1, 13.09.2013.

Research output: Contribution to journalArticle

Matsuoka, Ken ; Yamauchi, Noboru ; Gosho, Tadashi ; Asatuma, Satoru ; Toyooka, Kiminori ; Fujiwara, Toru. / Polarized localization and borate-dependent degradation of the Arabidopsis borate transporter BOR1 in tobacco BY-2 cells. In: F1000Research. 2013 ; Vol. 2.
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