Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma

Masaaki Yamamoto, Makoto Yamasaki, Yukiko Tsukao, Koji Tanaka, Yasuhiro Miyazaki, Tomoki Makino, Tsuyoshi Takahashi, Yukinori Kurokawa, Kiyokazu Nakajima, Shuji Takiguchi, Masaki Mori, Yuichiro Doki

Research output: Contribution to journalArticle

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Abstract

Poly (ADP-ribose) polymerase-1 (PARP1) plays a vital role in DNA repair and is expected to be an effective target in various malignancies. The aim of the present study was to investigate the clinical and biological significance of PARP1 expression in esophageal squamous cell carcinoma (ESCC). Immunohistochemical (IHC) staining was used to examine the association between PARP1 expression and the clinicopathological features of 86 patients with ESCC. The antitumor effect of small interfering RNA against PARP1 (siPARP1) was examined in a proliferation assay, and the mechanisms of this effect were investigated using western blot analysis and cell cycle assays. Cox multivariate analysis revealed that high expression of PARP1 in IHC staining was a statistically significant independent prognostic factor of poor overall survival (OS). The adjusted hazard ratio for OS in the group with high expression of PARP1 was 2.39 (95% confidence interval, 1.29-4.44; P=0.0051). In vitro assays showed that siPARP1 significantly decreased proliferation through G2/M arrest. Furthermore, western blot analysis showed that PARP1 was associated with the ataxia telangiectasia mutated-checkpoint kinase 2-cell division control 25c pathway. The present study suggests that PARP1 expression has a critical role in ESCC progression, and may be a clinical therapeutic target.

Original languageEnglish
Pages (from-to)1581-1587
Number of pages7
JournalOncology Letters
Volume14
Issue number2
DOIs
Publication statusPublished - Jan 1 2017

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Small Interfering RNA
Checkpoint Kinase 2
Western Blotting
Staining and Labeling
Ataxia Telangiectasia
Survival
Poly (ADP-Ribose) Polymerase-1
Esophageal Squamous Cell Carcinoma
DNA Repair
Cell Division
Cell Cycle
Multivariate Analysis
Confidence Intervals
Neoplasms
Therapeutics
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Yamamoto, M., Yamasaki, M., Tsukao, Y., Tanaka, K., Miyazaki, Y., Makino, T., ... Doki, Y. (2017). Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma. Oncology Letters, 14(2), 1581-1587. https://doi.org/10.3892/ol.2017.6334

Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma. / Yamamoto, Masaaki; Yamasaki, Makoto; Tsukao, Yukiko; Tanaka, Koji; Miyazaki, Yasuhiro; Makino, Tomoki; Takahashi, Tsuyoshi; Kurokawa, Yukinori; Nakajima, Kiyokazu; Takiguchi, Shuji; Mori, Masaki; Doki, Yuichiro.

In: Oncology Letters, Vol. 14, No. 2, 01.01.2017, p. 1581-1587.

Research output: Contribution to journalArticle

Yamamoto, M, Yamasaki, M, Tsukao, Y, Tanaka, K, Miyazaki, Y, Makino, T, Takahashi, T, Kurokawa, Y, Nakajima, K, Takiguchi, S, Mori, M & Doki, Y 2017, 'Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma', Oncology Letters, vol. 14, no. 2, pp. 1581-1587. https://doi.org/10.3892/ol.2017.6334
Yamamoto, Masaaki ; Yamasaki, Makoto ; Tsukao, Yukiko ; Tanaka, Koji ; Miyazaki, Yasuhiro ; Makino, Tomoki ; Takahashi, Tsuyoshi ; Kurokawa, Yukinori ; Nakajima, Kiyokazu ; Takiguchi, Shuji ; Mori, Masaki ; Doki, Yuichiro. / Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma. In: Oncology Letters. 2017 ; Vol. 14, No. 2. pp. 1581-1587.
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