Poly- and perfluoroalkyl substances in maternal serum: Method development and application in Pilot Study of the Japan Environment and Children's Study

Shoji F. Nakayama, Tomohiko Isobe, Miyuki Iwai-Shimada, Yayoi Kobayashi, Yukiko Nishihama, Yu Taniguchi, Makiko Sekiyama, Takehiro Michikawa, Shin Yamazaki, Hiroshi Nitta, Masako Oda, Hiroshi Mitsubuchi, Masafumi Sanefuji, Shouichi Ohga, Nathan Mise, Akihiko Ikegami, Reiko Suga, Masayuki Shimono

Research output: Contribution to journalArticle


Poly- and perfluoroalkyl substances (PFAS) have been investigated in a number of cohort studies due to concern over their adverse health effects. The aim of this study was to develop a reliable, high throughput and cost-effective analytical method for a broad range of PFAS in human serum. Protein precipitation, automatic solid phase extraction (SPE) pre-treatment and column-switching LC-MS/MS were employed. The optimised and validated method was then used to analyse the levels of 28 PFAS in 339 maternal serum samples from Pilot Study of the Japan Environment and Children's Study. Perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluoroundecanoic acid (PFUnA) and perfluorooctane sulphonic acid (PFOS) were detected in all 339 samples at median (range) concentrations of 1.9 (0.46–15), 1.5 (0.32–10), 1.3 (0.25–4.5) and 3.7 (0.43–15) ng/ml, respectively. These levels are comparable to those reported in previous studies using samples collected from various parts of the world. With a few exceptions, the remainder of the PFAS examined had lower detection rates but were found at concentrations similar to those reported in previous studies. The sensitivity and throughput ability of the method developed here are sufficient for its application in a large-scale biomonitoring study.

Original languageEnglish
Article number460933
JournalJournal of Chromatography A
Publication statusPublished - May 10 2020


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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