Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers

Jun Wang, Yong Zhang, Yeon Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A simple continuous isotachophoresis (ITP)-based microchip gel electrophoresis (MGE) was firstly applied for the concentration and separation of aptamer and its thrombin complex in a single cross-form poly (methyl methacrylate) (PMMA) microchip. The aptamer and its thrombin complex can be simultaneously concentrated and separated by this method which does not require time-consuming steps and complicated chip design. We found that different terminating electrolyte (TE) ions lead to different concentration enhancement factors and that lower mobility of TE ions can get higher concentration results. After optimization of various impact factors, we successfully achieved about 2000-fold concentration of aptamer-thrombin complex.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages791-793
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - Jan 1 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Fingerprint

Polymethyl Methacrylate
Electrophoresis
Polymethyl methacrylates
Buffers
Gels
Electrolytes
Ions
thrombin aptamer
Isotachophoresis

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Wang, J., Zhang, Y., Park, Y. S., Okamoto, Y., Kaji, N., Tokeshi, M., & Baba, Y. (2009). Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 791-793). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers. / Wang, Jun; Zhang, Yong; Park, Yeon Su; Okamoto, Yukihiro; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 791-793 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, J, Zhang, Y, Park, YS, Okamoto, Y, Kaji, N, Tokeshi, M & Baba, Y 2009, Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 791-793, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 11/1/09.
Wang J, Zhang Y, Park YS, Okamoto Y, Kaji N, Tokeshi M et al. Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 791-793. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Wang, Jun ; Zhang, Yong ; Park, Yeon Su ; Okamoto, Yukihiro ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 791-793 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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