Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish

Koji Yachiguchi, Noriko Matsumoto, Yuki Haga, Motoharu Suzuki, Chisato Matsumura, Masahiro Tsurukawa, Toshihiro Okuno, Takeshi Nakano, Kimi Kawabe, Kei ichiro Kitamura, Akira Toriba, Kazuichi Hayakawa, Vishwajit S. Chowdhury, Masato Endo, Atsuhiko Chiba, Toshio Sekiguchi, Masaki Nakano, Yoshiaki Tabuchi, Takashi Kondo, Shigehito WadaHiroyuki Mishima, Atsuhiko Hattori, Nobuo Suzuki

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Abstract

To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments.

Original languageEnglish
Pages (from-to)6365-6372
Number of pages8
JournalEnvironmental Science and Pollution Research
Volume21
Issue number10
DOIs
Publication statusPublished - May 2014

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All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., ... Suzuki, N. (2014). Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish. Environmental Science and Pollution Research, 21(10), 6365-6372. https://doi.org/10.1007/s11356-012-1347-5