TY - JOUR
T1 - Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish
AU - Yachiguchi, Koji
AU - Matsumoto, Noriko
AU - Haga, Yuki
AU - Suzuki, Motoharu
AU - Matsumura, Chisato
AU - Tsurukawa, Masahiro
AU - Okuno, Toshihiro
AU - Nakano, Takeshi
AU - Kawabe, Kimi
AU - Kitamura, Kei ichiro
AU - Toriba, Akira
AU - Hayakawa, Kazuichi
AU - Chowdhury, Vishwajit S.
AU - Endo, Masato
AU - Chiba, Atsuhiko
AU - Sekiguchi, Toshio
AU - Nakano, Masaki
AU - Tabuchi, Yoshiaki
AU - Kondo, Takashi
AU - Wada, Shigehito
AU - Mishima, Hiroyuki
AU - Hattori, Atsuhiko
AU - Suzuki, Nobuo
N1 - Funding Information:
Acknowledgments This study was supported in part by grants to N.S. [Kurita Water and Environment Foundation; Grant-in-Aid for Space Utilization by the Japan Aerospace Exploration Agency; Grant-in-Aid for Scientific Research (C) Nos. 21500404 and 24620004 by JSPS], to A.H. [Grant-in-Aid for Scientific Research (C) Nos. 21570062 and 24570068 by JSPS], to K.K. [Grant-in-Aid for Scientific Research (C) Nos. 21500681 and 24500848 by JSPS], to T.S. [Grant-in-Aid for Young Scientists (B) Nos. 22770069 and 40378568 by JSPS], to Y.T. [Grant-in-Aid for Scientific Research (B) No. 24310046 by JSPS], to T.N. [Grant-in-Aid for Scientific Research (B) No. 21310027 by JSPS], to H.M. [Grant-in-Aid for Scientific Research (C) No. 23592727 by JSPS], and to K.H. [the Environment Research and Technology Development Fund (B-0905) sponsored by the Ministry of the Environment, Japan; Health, Labor Sciences Research Grants of the Ministry of Health, Labor and Welfare, Japan; Grant-in-Aids for Scientific Research (B) No. 21390034 and for Exploratory Research No.24651044 by JSPS].
PY - 2014/5
Y1 - 2014/5
N2 - To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments.
AB - To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments.
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U2 - 10.1007/s11356-012-1347-5
DO - 10.1007/s11356-012-1347-5
M3 - Article
C2 - 23247518
AN - SCOPUS:84900842314
SN - 0944-1344
VL - 21
SP - 6365
EP - 6372
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
IS - 10
ER -