Polyclonal IgE induces mast cell survival and cytokine production

Jun Ichi Kashiwakura, Yuko Kawakami, Keisuke Yuki, Dirk M. Zajonc, Shunji Hasegawa, Yoshiaki Tomimori, Benjamin Caplan, Hirohisa Saito, Masutaka Furue, Hans C. Oettgen, Yoshimichi Okayama, Toshiaki Kawakami

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Ag-dependent activation of IgE-bearing mast cells is a critical first step in immediate hypersensitivity and other allergic responses. Recent studies have revealed Ag-independent effects of monoclonal mouse IgE molecules on mast cell survival and activation. However, no studies have been performed on the effects of polyclonal IgE molecules. Here, we tested whether polyclonal mouse and human IgE molecules affect survival and cytokine production in mast cells. Methods: Mast cells were cultured in the presence of polyclonal mouse and human IgE molecules, and cell survival and cytokine production were analyzed. Results: Polyclonal mouse IgE molecules in sera from mice with atopic dermatitis-like allergic skin inflammation, enhanced survival and cytokine production in mast cell cultures. Similar to the effects of monoclonal IgE, the polyclonal IgE effects were mediated by the high-affinity IgE receptor, FcεRI. Human polyclonal IgE molecules present in sera from atopic dermatitis patients were also capable of activating mast cells, and inducing IL-8 production in human cord blood-derived mast cells. Conclusions: These results imply that polyclonal IgE in atopic dermatitis and other atopic conditions might modulate mast cell number and function, thus amplifying the allergic response.

Original languageEnglish
Pages (from-to)411-419
Number of pages9
JournalAllergology International
Volume58
Issue number3
DOIs
Publication statusPublished - Jan 1 2009

Fingerprint

Mast Cells
Immunoglobulin E
Cell Survival
Cytokines
Atopic Dermatitis
IgE Receptors
Immediate Hypersensitivity
Survival
Serum
Interleukin-8
Fetal Blood
Cell Culture Techniques
Cell Count
Inflammation
Skin

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy

Cite this

Kashiwakura, J. I., Kawakami, Y., Yuki, K., Zajonc, D. M., Hasegawa, S., Tomimori, Y., ... Kawakami, T. (2009). Polyclonal IgE induces mast cell survival and cytokine production. Allergology International, 58(3), 411-419. https://doi.org/10.2332/allergolint.08-OA-0080

Polyclonal IgE induces mast cell survival and cytokine production. / Kashiwakura, Jun Ichi; Kawakami, Yuko; Yuki, Keisuke; Zajonc, Dirk M.; Hasegawa, Shunji; Tomimori, Yoshiaki; Caplan, Benjamin; Saito, Hirohisa; Furue, Masutaka; Oettgen, Hans C.; Okayama, Yoshimichi; Kawakami, Toshiaki.

In: Allergology International, Vol. 58, No. 3, 01.01.2009, p. 411-419.

Research output: Contribution to journalArticle

Kashiwakura, JI, Kawakami, Y, Yuki, K, Zajonc, DM, Hasegawa, S, Tomimori, Y, Caplan, B, Saito, H, Furue, M, Oettgen, HC, Okayama, Y & Kawakami, T 2009, 'Polyclonal IgE induces mast cell survival and cytokine production', Allergology International, vol. 58, no. 3, pp. 411-419. https://doi.org/10.2332/allergolint.08-OA-0080
Kashiwakura JI, Kawakami Y, Yuki K, Zajonc DM, Hasegawa S, Tomimori Y et al. Polyclonal IgE induces mast cell survival and cytokine production. Allergology International. 2009 Jan 1;58(3):411-419. https://doi.org/10.2332/allergolint.08-OA-0080
Kashiwakura, Jun Ichi ; Kawakami, Yuko ; Yuki, Keisuke ; Zajonc, Dirk M. ; Hasegawa, Shunji ; Tomimori, Yoshiaki ; Caplan, Benjamin ; Saito, Hirohisa ; Furue, Masutaka ; Oettgen, Hans C. ; Okayama, Yoshimichi ; Kawakami, Toshiaki. / Polyclonal IgE induces mast cell survival and cytokine production. In: Allergology International. 2009 ; Vol. 58, No. 3. pp. 411-419.
@article{c4098e9310014c449c10981de1f6aab1,
title = "Polyclonal IgE induces mast cell survival and cytokine production",
abstract = "Background: Ag-dependent activation of IgE-bearing mast cells is a critical first step in immediate hypersensitivity and other allergic responses. Recent studies have revealed Ag-independent effects of monoclonal mouse IgE molecules on mast cell survival and activation. However, no studies have been performed on the effects of polyclonal IgE molecules. Here, we tested whether polyclonal mouse and human IgE molecules affect survival and cytokine production in mast cells. Methods: Mast cells were cultured in the presence of polyclonal mouse and human IgE molecules, and cell survival and cytokine production were analyzed. Results: Polyclonal mouse IgE molecules in sera from mice with atopic dermatitis-like allergic skin inflammation, enhanced survival and cytokine production in mast cell cultures. Similar to the effects of monoclonal IgE, the polyclonal IgE effects were mediated by the high-affinity IgE receptor, FcεRI. Human polyclonal IgE molecules present in sera from atopic dermatitis patients were also capable of activating mast cells, and inducing IL-8 production in human cord blood-derived mast cells. Conclusions: These results imply that polyclonal IgE in atopic dermatitis and other atopic conditions might modulate mast cell number and function, thus amplifying the allergic response.",
author = "Kashiwakura, {Jun Ichi} and Yuko Kawakami and Keisuke Yuki and Zajonc, {Dirk M.} and Shunji Hasegawa and Yoshiaki Tomimori and Benjamin Caplan and Hirohisa Saito and Masutaka Furue and Oettgen, {Hans C.} and Yoshimichi Okayama and Toshiaki Kawakami",
year = "2009",
month = "1",
day = "1",
doi = "10.2332/allergolint.08-OA-0080",
language = "English",
volume = "58",
pages = "411--419",
journal = "Allergology International",
issn = "1323-8930",
publisher = "Japanese Society of Allergology",
number = "3",

}

TY - JOUR

T1 - Polyclonal IgE induces mast cell survival and cytokine production

AU - Kashiwakura, Jun Ichi

AU - Kawakami, Yuko

AU - Yuki, Keisuke

AU - Zajonc, Dirk M.

AU - Hasegawa, Shunji

AU - Tomimori, Yoshiaki

AU - Caplan, Benjamin

AU - Saito, Hirohisa

AU - Furue, Masutaka

AU - Oettgen, Hans C.

AU - Okayama, Yoshimichi

AU - Kawakami, Toshiaki

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Background: Ag-dependent activation of IgE-bearing mast cells is a critical first step in immediate hypersensitivity and other allergic responses. Recent studies have revealed Ag-independent effects of monoclonal mouse IgE molecules on mast cell survival and activation. However, no studies have been performed on the effects of polyclonal IgE molecules. Here, we tested whether polyclonal mouse and human IgE molecules affect survival and cytokine production in mast cells. Methods: Mast cells were cultured in the presence of polyclonal mouse and human IgE molecules, and cell survival and cytokine production were analyzed. Results: Polyclonal mouse IgE molecules in sera from mice with atopic dermatitis-like allergic skin inflammation, enhanced survival and cytokine production in mast cell cultures. Similar to the effects of monoclonal IgE, the polyclonal IgE effects were mediated by the high-affinity IgE receptor, FcεRI. Human polyclonal IgE molecules present in sera from atopic dermatitis patients were also capable of activating mast cells, and inducing IL-8 production in human cord blood-derived mast cells. Conclusions: These results imply that polyclonal IgE in atopic dermatitis and other atopic conditions might modulate mast cell number and function, thus amplifying the allergic response.

AB - Background: Ag-dependent activation of IgE-bearing mast cells is a critical first step in immediate hypersensitivity and other allergic responses. Recent studies have revealed Ag-independent effects of monoclonal mouse IgE molecules on mast cell survival and activation. However, no studies have been performed on the effects of polyclonal IgE molecules. Here, we tested whether polyclonal mouse and human IgE molecules affect survival and cytokine production in mast cells. Methods: Mast cells were cultured in the presence of polyclonal mouse and human IgE molecules, and cell survival and cytokine production were analyzed. Results: Polyclonal mouse IgE molecules in sera from mice with atopic dermatitis-like allergic skin inflammation, enhanced survival and cytokine production in mast cell cultures. Similar to the effects of monoclonal IgE, the polyclonal IgE effects were mediated by the high-affinity IgE receptor, FcεRI. Human polyclonal IgE molecules present in sera from atopic dermatitis patients were also capable of activating mast cells, and inducing IL-8 production in human cord blood-derived mast cells. Conclusions: These results imply that polyclonal IgE in atopic dermatitis and other atopic conditions might modulate mast cell number and function, thus amplifying the allergic response.

UR - http://www.scopus.com/inward/record.url?scp=70449578104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70449578104&partnerID=8YFLogxK

U2 - 10.2332/allergolint.08-OA-0080

DO - 10.2332/allergolint.08-OA-0080

M3 - Article

C2 - 19542764

AN - SCOPUS:70449578104

VL - 58

SP - 411

EP - 419

JO - Allergology International

JF - Allergology International

SN - 1323-8930

IS - 3

ER -