Abstract
In mammals, germ cells undergo a series of events to give rise to haploid gametes. The dynamics of meiotic chromatin architecture still remain poorly understood. By applying Hi-C to mouse oocytes, Du et al. reveal a unique Polycomb-regulated chromatin architecture, which adds a new perspective to the non-canonical oocyte epigenomes.
| Original language | English |
|---|---|
| Pages (from-to) | 825-839.e7 |
| Journal | Molecular Cell |
| Volume | 77 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Feb 20 2020 |
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
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Polycomb Group Proteins Regulate Chromatin Architecture in Mouse Oocytes and Early Embryos. / Du, Zhenhai; Zheng, Hui; Kawamura, Yumiko K.; Zhang, Ke; Gassler, Johanna; Powell, Sean; Xu, Qianhua; Lin, Zili; Xu, Kai; Zhou, Qian; Ozonov, Evgeniy A.; Véron, Nathalie; Huang, Bo; Li, Lijia; Yu, Guang; Liu, Ling; Au Yeung, Wan Kin; Wang, Peizhe; Chang, Lei; Wang, Qiujun; He, Aibin; Sun, Yujie; Na, Jie; Sun, Qingyuan; Sasaki, Hiroyuki; Tachibana, Kikuë; Peters, Antoine H.F.M.; Xie, Wei.
In: Molecular Cell, Vol. 77, No. 4, 20.02.2020, p. 825-839.e7.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Polycomb Group Proteins Regulate Chromatin Architecture in Mouse Oocytes and Early Embryos
AU - Du, Zhenhai
AU - Zheng, Hui
AU - Kawamura, Yumiko K.
AU - Zhang, Ke
AU - Gassler, Johanna
AU - Powell, Sean
AU - Xu, Qianhua
AU - Lin, Zili
AU - Xu, Kai
AU - Zhou, Qian
AU - Ozonov, Evgeniy A.
AU - Véron, Nathalie
AU - Huang, Bo
AU - Li, Lijia
AU - Yu, Guang
AU - Liu, Ling
AU - Au Yeung, Wan Kin
AU - Wang, Peizhe
AU - Chang, Lei
AU - Wang, Qiujun
AU - He, Aibin
AU - Sun, Yujie
AU - Na, Jie
AU - Sun, Qingyuan
AU - Sasaki, Hiroyuki
AU - Tachibana, Kikuë
AU - Peters, Antoine H.F.M.
AU - Xie, Wei
N1 - Funding Information: We thank Prof. Fei Gao (IOZ, Beijing, China) for providing Gdf9-Cre mice, Dr. Miguel Vidal (CSIC, Madrid, Spain) for Ring1 deficient mice, Prof. Maarten van Lohuizen (NKI, Amsterdam, the Netherlands) for Rnf2fl/fl mice, and Prof. Barbara Knowles (JAX, Bar Harbor, ME, USA) for Zp3-Cre transgenic mice. We are grateful to Rui Ma, Hui Quan, Xianglin Zhang, Zhaofeng Ye, Yuanyuan Li, Wenhao Zhang, Xiaowo Wang, Jianyang Zeng, Yiqin Gao, and Juntao Gao for discussions. We thank Drs. Haruhiko Koseki, Yi Zhang, and Inoue Azusa for sharing reagents or protocols. We appreciate members of the Xie laboratory for comments during preparation of the manuscript. This work is supported by funding provided by the National Natural Science Foundation of China (grants 31422031 and 31725018 to W.X.); the National Basic Research Program of China (grant 2015CB856201 to W.X.); the Beijing Municipal Science & Technology Commission (grant Z181100001318006 to W.X.); the THU-PKU Center for Life Sciences (W.X.); the Novartis Research Foundation (N.V. and A.H.F.M.P.); the European Research Council (grant ERC 695288?Totipotency to Y.K.K. E.A.O. and A.H.F.M.P.); and the JSPS (KAKENHI JP18H05214 to H.S.). Research in the laboratory of K.T. is supported by the Austrian Academy of Sciences, the FWF and Herzfelder foundation (P30613-B21), a European Research Council grant (ERC-StG-336460 ChromHeritance), and an HFSP project grant (PGP0057-2018). Z.D. H.Z. and Q.X. are supported by THU-PKU Center for Life Sciences postdoctoral fellowships. W.X. is an HHMI international research scholar. J.G. is supported by the European Research Council and the L'Or?al Austria Fellowship for Women in Science and is an associated student of the DK Chromosome Dynamics (W1238-B20) supported by the Austrian Science Fund (FWF). Z.D. H.Z. and W.X. conceived the project. Z.D. conducted the sisHi-C experiments. Z.L. Q.X. B.H. K.X. Z.D. Y.K.K. J.G. and K.Z. performed the mouse PGCs, oocytes, and embryo experiments. H.Z. and Z.D. performed bioinformatics analysis. L. Li and G.Y. performed the CUT&RUN experiments. J.G. performed the single-nucleus Hi-C experiments. S.P. processed the single-nucleus Hi-C data. L.C. P.W. L. Liu, E.A.O. N.V. Q.Z. and W.K.A.Y. helped with various experiments or analyses. Q.W. performed next-generation sequencing (NGS). A.H. Y.S. J.N. Q.S. H.S. K.T. A.H.F.M.P. and W.X. supervised the project or related experiments. Z.D. H.Z. K.Z. A.H.F.M.P. and W.X. wrote the manuscript, with help from other authors. The authors declare no competing interests. Funding Information: We thank Prof. Fei Gao (IOZ, Beijing, China) for providing Gdf9-Cre mice, Dr. Miguel Vidal (CSIC, Madrid, Spain) for Ring1 deficient mice, Prof. Maarten van Lohuizen (NKI, Amsterdam, the Netherlands) for Rnf2 fl/fl mice, and Prof. Barbara Knowles (JAX, Bar Harbor, ME, USA) for Zp3-Cre transgenic mice. We are grateful to Rui Ma, Hui Quan, Xianglin Zhang, Zhaofeng Ye, Yuanyuan Li, Wenhao Zhang, Xiaowo Wang, Jianyang Zeng, Yiqin Gao, and Juntao Gao for discussions. We thank Drs. Haruhiko Koseki, Yi Zhang, and Inoue Azusa for sharing reagents or protocols. We appreciate members of the Xie laboratory for comments during preparation of the manuscript. This work is supported by funding provided by the National Natural Science Foundation of China (grants 31422031 and 31725018 to W.X.); the National Basic Research Program of China (grant 2 015CB856201 to W.X.); the Beijing Municipal Science & Technology Commission (grant Z181100001318006 to W.X.); the THU-PKU Center for Life Sciences (W.X.); the Novartis Research Foundation (N.V. and A.H.F.M.P.); the European Research Council (grant ERC 695288–Totipotency to Y.K.K., E.A.O., and A.H.F.M.P.); and the JSPS ( KAKENHI JP18H05214 to H.S.). Research in the laboratory of K.T. is supported by the Austrian Academy of Sciences , the FWF and Herzfelder foundation ( P30613-B21 ), a European Research Council grant ( ERC-StG-336460 ChromHeritance), and an HFSP project grant ( PGP0057-2018 ). Z.D., H.Z., and Q.X. are supported by THU-PKU Center for Life Sciences postdoctoral fellowships. W.X. is an HHMI international research scholar. J.G. is supported by the European Research Council and the L'Oréal Austria Fellowship for Women in Science and is an associated student of the DK Chromosome Dynamics ( W1238-B20 ) supported by the Austrian Science Fund (FWF) . Publisher Copyright: © 2019 Elsevier Inc.
PY - 2020/2/20
Y1 - 2020/2/20
N2 - In mammals, germ cells undergo a series of events to give rise to haploid gametes. The dynamics of meiotic chromatin architecture still remain poorly understood. By applying Hi-C to mouse oocytes, Du et al. reveal a unique Polycomb-regulated chromatin architecture, which adds a new perspective to the non-canonical oocyte epigenomes.
AB - In mammals, germ cells undergo a series of events to give rise to haploid gametes. The dynamics of meiotic chromatin architecture still remain poorly understood. By applying Hi-C to mouse oocytes, Du et al. reveal a unique Polycomb-regulated chromatin architecture, which adds a new perspective to the non-canonical oocyte epigenomes.
UR - http://www.scopus.com/inward/record.url?scp=85079365044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079365044&partnerID=8YFLogxK
U2 - 10.1016/j.molcel.2019.11.011
DO - 10.1016/j.molcel.2019.11.011
M3 - Article
C2 - 31837995
AN - SCOPUS:85079365044
VL - 77
SP - 825-839.e7
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 4
ER -