Polyethylene oxide (PEO) and polyethylene glycol (PEG) polymer sieving matrix for RNA capillary electrophoresis

Yoshinori Yamaguchi; Zhenqing Li; Xifang Zhu; Chenchen Liu; Dawei Zhang; Xiaoming Dou

doi:10.1371/journal.pone.0123406

Polyethylene oxide (PEO) and polyethylene glycol (PEG) polymer sieving matrix for RNA capillary electrophoresis

Yoshinori Yamaguchi, Zhenqing Li, Xifang Zhu, Chenchen Liu, Dawei Zhang, Xiaoming Dou

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (N_min) from the resolution length analysis.

Original language	English
Article number	e0123406
Journal	PloS one
Volume	10
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0123406
Publication status	Published - May 1 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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abstract = "The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (Nmin) from the resolution length analysis.",

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AU - Yamaguchi, Yoshinori

AU - Li, Zhenqing

AU - Zhu, Xifang

AU - Liu, Chenchen

AU - Zhang, Dawei

AU - Dou, Xiaoming

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AB - The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (Nmin) from the resolution length analysis.

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Polyethylene oxide (PEO) and polyethylene glycol (PEG) polymer sieving matrix for RNA capillary electrophoresis

Abstract

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