Polyethylene oxide (PEO) and polyethylene glycol (PEG) polymer sieving matrix for RNA capillary electrophoresis

Yoshinori Yamaguchi, Zhenqing Li, Xifang Zhu, Chenchen Liu, Dawei Zhang, Xiaoming Dou

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The selection of sieving polymer for RNA fragments separation by capillary electrophoresis is imperative. We investigated the separation of RNA fragments ranged from 100 to 10,000 nt in polyethylene glycol (PEG) and polyethylene oxide (PEO) solutions with different molecular weight and different concentration. We found that the separation performance of the small RNA fragments (4000 nt) deteriorated in PEG/PEO solutions when the concentration was above 1.0%/0.6%, respectively. By double logarithmic plot of mobility and RNA fragment size, we revealed three migration regimes for RNA in PEG (300-500k) and PEO (4,000k). Moreover, we calculated the smallest resolvable nucleotide length (Nmin) from the resolution length analysis.

Original languageEnglish
Article numbere0123406
JournalPloS one
Issue number5
Publication statusPublished - May 1 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General


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