Random copolymers (P(M100-m/T m )) composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), which suppresses protein adsorption, and 3-(triethoxysilyl)propyl methacrylate (MTEOS), which can be covalently fixed on a glass surface, were prepared via photoinitiated radical polymerization. When P(M100-m/T m ) was coated on a glass surface, a protein antifouling effect could be observed because of the presence of MPC units on the glass surface. To confirm the coating of the glass surface with P(M100-m/T m ) by fluorescence microscopy, pyrene-labeled P(M100-m/T m ) was also prepared. An ethanol solution of P(M100-m/T m ) was spin-coated on the glass, which was exposed to NH3 vapor to promote the reaction of the pendant triethoxysilyl groups in P(M100-m/T m ) with silanol groups on the glass. The coating of the glass with MPC was confirmed by fluorescence microscopy. The protein antifouling effects of the P(M100-m/T m )-coated glass were confirmed using fluorescence-labeled proteins. It is expected that P(M100-m/T m ) can be applied as a surface-coating agent on glass containers for protein formulations.
All Science Journal Classification (ASJC) codes
- Polymers and Plastics
- Materials Chemistry