Polymer coating glass to improve the protein antifouling effect

Tomoko Honda, Aiko Nakao, Kazuhiko Ishihara, Yuji Higaki, Keiko Higaki, Atsushi Takahara, Yasuhiko Iwasaki, Shin Ichi Yusa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Random copolymers (P(M100-m/T m )) composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), which suppresses protein adsorption, and 3-(triethoxysilyl)propyl methacrylate (MTEOS), which can be covalently fixed on a glass surface, were prepared via photoinitiated radical polymerization. When P(M100-m/T m ) was coated on a glass surface, a protein antifouling effect could be observed because of the presence of MPC units on the glass surface. To confirm the coating of the glass surface with P(M100-m/T m ) by fluorescence microscopy, pyrene-labeled P(M100-m/T m ) was also prepared. An ethanol solution of P(M100-m/T m ) was spin-coated on the glass, which was exposed to NH3 vapor to promote the reaction of the pendant triethoxysilyl groups in P(M100-m/T m ) with silanol groups on the glass. The coating of the glass with MPC was confirmed by fluorescence microscopy. The protein antifouling effects of the P(M100-m/T m )-coated glass were confirmed using fluorescence-labeled proteins. It is expected that P(M100-m/T m ) can be applied as a surface-coating agent on glass containers for protein formulations.

Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalPolymer Journal
Volume50
Issue number5
DOIs
Publication statusPublished - May 1 2018

Fingerprint

Polymers
Proteins
Glass
Coatings
Phosphorylcholine
Fluorescence microscopy
Methacrylates
Pyrene
Free radical polymerization
Containers
Ethanol
Copolymers
Fluorescence
Vapors
Adsorption

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Honda, T., Nakao, A., Ishihara, K., Higaki, Y., Higaki, K., Takahara, A., ... Yusa, S. I. (2018). Polymer coating glass to improve the protein antifouling effect. Polymer Journal, 50(5), 381-388. https://doi.org/10.1038/s41428-018-0026-x

Polymer coating glass to improve the protein antifouling effect. / Honda, Tomoko; Nakao, Aiko; Ishihara, Kazuhiko; Higaki, Yuji; Higaki, Keiko; Takahara, Atsushi; Iwasaki, Yasuhiko; Yusa, Shin Ichi.

In: Polymer Journal, Vol. 50, No. 5, 01.05.2018, p. 381-388.

Research output: Contribution to journalArticle

Honda, T, Nakao, A, Ishihara, K, Higaki, Y, Higaki, K, Takahara, A, Iwasaki, Y & Yusa, SI 2018, 'Polymer coating glass to improve the protein antifouling effect', Polymer Journal, vol. 50, no. 5, pp. 381-388. https://doi.org/10.1038/s41428-018-0026-x
Honda, Tomoko ; Nakao, Aiko ; Ishihara, Kazuhiko ; Higaki, Yuji ; Higaki, Keiko ; Takahara, Atsushi ; Iwasaki, Yasuhiko ; Yusa, Shin Ichi. / Polymer coating glass to improve the protein antifouling effect. In: Polymer Journal. 2018 ; Vol. 50, No. 5. pp. 381-388.
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