Polymer coating glass to improve the protein antifouling effect

Tomoko Honda, Aiko Nakao, Kazuhiko Ishihara, Yuji Higaki, Keiko Higaki, Atsushi Takahara, Yasuhiko Iwasaki, Shin Ichi Yusa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Random copolymers (P(M100-m/T m )) composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), which suppresses protein adsorption, and 3-(triethoxysilyl)propyl methacrylate (MTEOS), which can be covalently fixed on a glass surface, were prepared via photoinitiated radical polymerization. When P(M100-m/T m ) was coated on a glass surface, a protein antifouling effect could be observed because of the presence of MPC units on the glass surface. To confirm the coating of the glass surface with P(M100-m/T m ) by fluorescence microscopy, pyrene-labeled P(M100-m/T m ) was also prepared. An ethanol solution of P(M100-m/T m ) was spin-coated on the glass, which was exposed to NH3 vapor to promote the reaction of the pendant triethoxysilyl groups in P(M100-m/T m ) with silanol groups on the glass. The coating of the glass with MPC was confirmed by fluorescence microscopy. The protein antifouling effects of the P(M100-m/T m )-coated glass were confirmed using fluorescence-labeled proteins. It is expected that P(M100-m/T m ) can be applied as a surface-coating agent on glass containers for protein formulations.

Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalPolymer Journal
Volume50
Issue number5
DOIs
Publication statusPublished - May 1 2018

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

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  • Cite this

    Honda, T., Nakao, A., Ishihara, K., Higaki, Y., Higaki, K., Takahara, A., Iwasaki, Y., & Yusa, S. I. (2018). Polymer coating glass to improve the protein antifouling effect. Polymer Journal, 50(5), 381-388. https://doi.org/10.1038/s41428-018-0026-x