Polymer-modified gold nanoparticles via RAFT polymerization

A detailed study for a biosensing application

Masaki Takara, Masayuki Toyoshima, Hirokazu Seto, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Glycopolymers of polyacrylamide derivatives with mannose were prepared via the living radical polymerization of a reversible addition-fragmentation chain transfer reagent. The polymers obtained showed narrow polydispersities. The polymer terminal group was reduced to a thiol, and the resulting polymers were mixed with gold nanoparticles to prepare glycopolymer-substituted gold nanoparticles. The mannose density was adjusted by varying the copolymer preparation and the glycopolymer-polyacrylamide mixture. The colloidal stability of the polymer-coated gold nanoparticles is dependent on the mannose density. Polymer-coated nanoparticles with low mannose densities showed better colloidal stabilities. The molecular recognition abilities of the polymer were investigated using UV-vis spectroscopy. The polymer-coated nanoparticles showed strong protein recognition abilities because of multivalent binding effects. Polymers with high mannose densities showed stronger recognition abilities. The molecular recognition abilities of the glycopolymer-polyacrylamide mixed nanoparticles are dependent on the mannose density. An immunochromatographic assay was performed using the polymer-coated nanoparticles. The color was detected from the gold nanoparticles in the nanoparticle systems with strong molecular recognition and good colloid stability.

Original languageEnglish
Pages (from-to)931-939
Number of pages9
JournalPolymer Chemistry
Volume5
Issue number3
DOIs
Publication statusPublished - Feb 7 2014

Fingerprint

Polymerization
Gold
Nanoparticles
Polymers
Mannose
Molecular recognition
Polyacrylates
Immunochromatography
Living polymerization
Colloids
Polydispersity
Free radical polymerization
Ultraviolet spectroscopy
Sulfhydryl Compounds
Assays
Spectrum Analysis
Copolymers
Color
Derivatives
Proteins

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Polymers and Plastics
  • Organic Chemistry

Cite this

Polymer-modified gold nanoparticles via RAFT polymerization : A detailed study for a biosensing application. / Takara, Masaki; Toyoshima, Masayuki; Seto, Hirokazu; Hoshino, Yu; Miura, Yoshiko.

In: Polymer Chemistry, Vol. 5, No. 3, 07.02.2014, p. 931-939.

Research output: Contribution to journalArticle

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