Abstract
Glycopolymers of polyacrylamide derivatives with mannose were prepared via the living radical polymerization of a reversible addition-fragmentation chain transfer reagent. The polymers obtained showed narrow polydispersities. The polymer terminal group was reduced to a thiol, and the resulting polymers were mixed with gold nanoparticles to prepare glycopolymer-substituted gold nanoparticles. The mannose density was adjusted by varying the copolymer preparation and the glycopolymer-polyacrylamide mixture. The colloidal stability of the polymer-coated gold nanoparticles is dependent on the mannose density. Polymer-coated nanoparticles with low mannose densities showed better colloidal stabilities. The molecular recognition abilities of the polymer were investigated using UV-vis spectroscopy. The polymer-coated nanoparticles showed strong protein recognition abilities because of multivalent binding effects. Polymers with high mannose densities showed stronger recognition abilities. The molecular recognition abilities of the glycopolymer-polyacrylamide mixed nanoparticles are dependent on the mannose density. An immunochromatographic assay was performed using the polymer-coated nanoparticles. The color was detected from the gold nanoparticles in the nanoparticle systems with strong molecular recognition and good colloid stability.
| Original language | English |
|---|---|
| Pages (from-to) | 931-939 |
| Number of pages | 9 |
| Journal | Polymer Chemistry |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Feb 7 2014 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Biochemistry
- Polymers and Plastics
- Organic Chemistry
Cite this
Polymer-modified gold nanoparticles via RAFT polymerization : A detailed study for a biosensing application. / Takara, Masaki; Toyoshima, Masayuki; Seto, Hirokazu; Hoshino, Yu; Miura, Yoshiko.
In: Polymer Chemistry, Vol. 5, No. 3, 07.02.2014, p. 931-939.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polymer-modified gold nanoparticles via RAFT polymerization
T2 - A detailed study for a biosensing application
AU - Takara, Masaki
AU - Toyoshima, Masayuki
AU - Seto, Hirokazu
AU - Hoshino, Yu
AU - Miura, Yoshiko
PY - 2014/2/7
Y1 - 2014/2/7
N2 - Glycopolymers of polyacrylamide derivatives with mannose were prepared via the living radical polymerization of a reversible addition-fragmentation chain transfer reagent. The polymers obtained showed narrow polydispersities. The polymer terminal group was reduced to a thiol, and the resulting polymers were mixed with gold nanoparticles to prepare glycopolymer-substituted gold nanoparticles. The mannose density was adjusted by varying the copolymer preparation and the glycopolymer-polyacrylamide mixture. The colloidal stability of the polymer-coated gold nanoparticles is dependent on the mannose density. Polymer-coated nanoparticles with low mannose densities showed better colloidal stabilities. The molecular recognition abilities of the polymer were investigated using UV-vis spectroscopy. The polymer-coated nanoparticles showed strong protein recognition abilities because of multivalent binding effects. Polymers with high mannose densities showed stronger recognition abilities. The molecular recognition abilities of the glycopolymer-polyacrylamide mixed nanoparticles are dependent on the mannose density. An immunochromatographic assay was performed using the polymer-coated nanoparticles. The color was detected from the gold nanoparticles in the nanoparticle systems with strong molecular recognition and good colloid stability.
AB - Glycopolymers of polyacrylamide derivatives with mannose were prepared via the living radical polymerization of a reversible addition-fragmentation chain transfer reagent. The polymers obtained showed narrow polydispersities. The polymer terminal group was reduced to a thiol, and the resulting polymers were mixed with gold nanoparticles to prepare glycopolymer-substituted gold nanoparticles. The mannose density was adjusted by varying the copolymer preparation and the glycopolymer-polyacrylamide mixture. The colloidal stability of the polymer-coated gold nanoparticles is dependent on the mannose density. Polymer-coated nanoparticles with low mannose densities showed better colloidal stabilities. The molecular recognition abilities of the polymer were investigated using UV-vis spectroscopy. The polymer-coated nanoparticles showed strong protein recognition abilities because of multivalent binding effects. Polymers with high mannose densities showed stronger recognition abilities. The molecular recognition abilities of the glycopolymer-polyacrylamide mixed nanoparticles are dependent on the mannose density. An immunochromatographic assay was performed using the polymer-coated nanoparticles. The color was detected from the gold nanoparticles in the nanoparticle systems with strong molecular recognition and good colloid stability.
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U2 - 10.1039/c3py01001e
DO - 10.1039/c3py01001e
M3 - Article
VL - 5
SP - 931
EP - 939
JO - Polymer Chemistry
JF - Polymer Chemistry
SN - 1759-9954
IS - 3
ER -