Polymer nanoparticle-protein interface. Evaluation of the contribution of positively charged functional groups to protein affinity

Yusuke Yonamine, Keiichi Yoshimatsu, Shih Hui Lee, Yu Hoshino, Yoshio Okahata, Kenneth J. Shea

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Cationic-functionalized polymer nanoparticles (NPs) show strikingly distinct affinities to proteins depending on the nature of the cationic functional group. N-Isopropylacrylamide (NIPAm) polymer NPs incorporating three types of positively charged functional groups (guanidinium, primary amino, and quaternary ammonium groups) were prepared by precipitation polymerization. The affinities to fibrinogen, a protein with an isoelectric point (pI) of 5.5, were compared using UV-vis spectrometry and a quartz crystal microbalance (QCM). Guanidinium-containing NPs showed the highest affinity to fibrinogen. The observation is attributed to strong, specific interactions with carboxylate groups on the protein surface. The affinity of the positively charged NPs to proteins with a range of pIs revealed that protein-NP affinity is due to a combination of ionic, hydrogen bonding, and hydrophobic interactions. Protein affinity can be modulated by varying the composition of these functional monomers in the acrylamide NPs. Engineered NPs containing the guanidinium group with hydrophobic and hydrogen bonding functional groups were used in an affinity precipitation for the selective separation of fibrinogen from a plasma protein mixture. Circular dichroism (CD) revealed that the protein was not denatured in the process of binding or release.

Original languageEnglish
Pages (from-to)374-379
Number of pages6
JournalACS Applied Materials and Interfaces
Volume5
Issue number2
DOIs
Publication statusPublished - Jan 23 2013

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Functional groups
Polymers
Nanoparticles
Proteins
Guanidine
Fibrinogen
Hydrogen bonds
Acrylamide
Quartz crystal microbalances
Ammonium Compounds
Dichroism
Spectrometry
Blood Proteins
Membrane Proteins
Monomers
Polymerization
Plasmas
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Polymer nanoparticle-protein interface. Evaluation of the contribution of positively charged functional groups to protein affinity. / Yonamine, Yusuke; Yoshimatsu, Keiichi; Lee, Shih Hui; Hoshino, Yu; Okahata, Yoshio; Shea, Kenneth J.

In: ACS Applied Materials and Interfaces, Vol. 5, No. 2, 23.01.2013, p. 374-379.

Research output: Contribution to journalArticle

Yonamine, Yusuke ; Yoshimatsu, Keiichi ; Lee, Shih Hui ; Hoshino, Yu ; Okahata, Yoshio ; Shea, Kenneth J. / Polymer nanoparticle-protein interface. Evaluation of the contribution of positively charged functional groups to protein affinity. In: ACS Applied Materials and Interfaces. 2013 ; Vol. 5, No. 2. pp. 374-379.
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