Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood

Kosei Moriyama, Chie Ando, Kosuke Tashiro, Satoru Kuhara, Seiichi Okamura, Shuji Nakano, Yasumitsu Takagi, Takeyoshi Miki, Yoshiyuki Nakashima, Hideki Hirakawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples,PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.

Original languageEnglish
Pages (from-to)375-382
Number of pages8
JournalMICROBIOLOGY and IMMUNOLOGY
Volume52
Issue number7
DOIs
Publication statusPublished - Jul 1 2008

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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