Poly(methylmethacrylate) microchip electrophoresis of proteins using linear-poly(acrylamide) solutions as separation matrix

Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed using the MC-coated microchips with Ferguson plot-fittings. The entangled L-PA solution provided high resolution of peaks of SDS-PC when the concentration of L-PA was increased. Some investigations into the separation mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus the logarithm of the molecular weight of the complex exhibiting linear behavior, indicated that the separation mechanism was dependent on mass discrimination, in accordance with Ogston model. 2008

Original languageEnglish
Pages (from-to)321-325
Number of pages5
Journalanalytical sciences
Volume24
Issue number3
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Poly(methylmethacrylate) microchip electrophoresis of proteins using linear-poly(acrylamide) solutions as separation matrix'. Together they form a unique fingerprint.

Cite this