Abstract
Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed using the MC-coated microchips with Ferguson plot-fittings. The entangled L-PA solution provided high resolution of peaks of SDS-PC when the concentration of L-PA was increased. Some investigations into the separation mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus the logarithm of the molecular weight of the complex exhibiting linear behavior, indicated that the separation mechanism was dependent on mass discrimination, in accordance with Ogston model. 2008
Original language | English |
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Pages (from-to) | 321-325 |
Number of pages | 5 |
Journal | analytical sciences |
Volume | 24 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2008 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry