Polymorphism in the upstream regulatory region of DQA1 genes and DRB1, QAP, DQA1, and DQB1 haplotypes in the German population

Johannes Peter Haas, Akinori Kimura, Adriane Andreas, Martina Hochberger, Elisabeth Keller, Günther Brünnler, Maria de la Paz Bettinotti, Claudia Nevinny-Stickel, Bernhard Hildebrandt, Gabriele Sierp, Takehiko Sasazuki, Ekkehard Albert

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Abstract

Polymorphism in the URR of the MHC class II DQA1 gene defines ten different alleles named QAP. Oligotyping for the alleles of DRB1, QAP, DQA1, and DQB1 have been performed in 210 unrelated healthy controls from Germany. Moreover, 83 HTCs from the Tenth IHWS have been tested. Four point loci haplotypes (DRB1, QAP, DQA1, and DQB1) have been analyzed in the unrelated healthy population sample. Computer analysis of the linkage disequilibria leads to the conclusion that QAP alleles are in strong linkage disequilibrium with alleles either the DQA1 or the DRB1 locus. One typical ("common") haplotype was found to be associated with each DRB1 allele in the majority (86%) of the tested persons. Apart from that, 25 other less frequent ("unusual") haplotypes, with an overall frequency of 14% have been defined. Some of these "unusual" MHC class II haplotypes were found to differ only in the regulatory alleles of DQA1 (QAP alleles) while they are identical for the alleles coding for structural elements (DRB1, DQA1, and DQB1). Most of the "unusual" haplotypes were found to carry HLA-DQ6. Assuming that "unusual" (= rare) haplotypes have arisen from "common" (= frequent) haplotypes by point mutation and recombination, we propose the existence of three recombination sites in the MHC DR-DQ region: one between DRB1 and QAP, the second between QAP and DQA1, and the third between DQA1 and DQB1.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalHuman Immunology
Volume39
Issue number1
DOIs
Publication statusPublished - Jan 1 1994

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Nucleic Acid Regulatory Sequences
Haplotypes
Alleles
Population
Genes
Linkage Disequilibrium
Genetic Recombination
MHC Class II Genes
Point Mutation
Germany

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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Polymorphism in the upstream regulatory region of DQA1 genes and DRB1, QAP, DQA1, and DQB1 haplotypes in the German population. / Haas, Johannes Peter; Kimura, Akinori; Andreas, Adriane; Hochberger, Martina; Keller, Elisabeth; Brünnler, Günther; de la Paz Bettinotti, Maria; Nevinny-Stickel, Claudia; Hildebrandt, Bernhard; Sierp, Gabriele; Sasazuki, Takehiko; Albert, Ekkehard.

In: Human Immunology, Vol. 39, No. 1, 01.01.1994, p. 31-40.

Research output: Contribution to journalArticle

Haas, JP, Kimura, A, Andreas, A, Hochberger, M, Keller, E, Brünnler, G, de la Paz Bettinotti, M, Nevinny-Stickel, C, Hildebrandt, B, Sierp, G, Sasazuki, T & Albert, E 1994, 'Polymorphism in the upstream regulatory region of DQA1 genes and DRB1, QAP, DQA1, and DQB1 haplotypes in the German population', Human Immunology, vol. 39, no. 1, pp. 31-40. https://doi.org/10.1016/0198-8859(94)90098-1
Haas, Johannes Peter ; Kimura, Akinori ; Andreas, Adriane ; Hochberger, Martina ; Keller, Elisabeth ; Brünnler, Günther ; de la Paz Bettinotti, Maria ; Nevinny-Stickel, Claudia ; Hildebrandt, Bernhard ; Sierp, Gabriele ; Sasazuki, Takehiko ; Albert, Ekkehard. / Polymorphism in the upstream regulatory region of DQA1 genes and DRB1, QAP, DQA1, and DQB1 haplotypes in the German population. In: Human Immunology. 1994 ; Vol. 39, No. 1. pp. 31-40.
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AU - Haas, Johannes Peter

AU - Kimura, Akinori

AU - Andreas, Adriane

AU - Hochberger, Martina

AU - Keller, Elisabeth

AU - Brünnler, Günther

AU - de la Paz Bettinotti, Maria

AU - Nevinny-Stickel, Claudia

AU - Hildebrandt, Bernhard

AU - Sierp, Gabriele

AU - Sasazuki, Takehiko

AU - Albert, Ekkehard

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N2 - Polymorphism in the URR of the MHC class II DQA1 gene defines ten different alleles named QAP. Oligotyping for the alleles of DRB1, QAP, DQA1, and DQB1 have been performed in 210 unrelated healthy controls from Germany. Moreover, 83 HTCs from the Tenth IHWS have been tested. Four point loci haplotypes (DRB1, QAP, DQA1, and DQB1) have been analyzed in the unrelated healthy population sample. Computer analysis of the linkage disequilibria leads to the conclusion that QAP alleles are in strong linkage disequilibrium with alleles either the DQA1 or the DRB1 locus. One typical ("common") haplotype was found to be associated with each DRB1 allele in the majority (86%) of the tested persons. Apart from that, 25 other less frequent ("unusual") haplotypes, with an overall frequency of 14% have been defined. Some of these "unusual" MHC class II haplotypes were found to differ only in the regulatory alleles of DQA1 (QAP alleles) while they are identical for the alleles coding for structural elements (DRB1, DQA1, and DQB1). Most of the "unusual" haplotypes were found to carry HLA-DQ6. Assuming that "unusual" (= rare) haplotypes have arisen from "common" (= frequent) haplotypes by point mutation and recombination, we propose the existence of three recombination sites in the MHC DR-DQ region: one between DRB1 and QAP, the second between QAP and DQA1, and the third between DQA1 and DQB1.

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