Polymorphism of T-cell receptor genes among laboratory and wild mice: Diverse origins of laboratory mice

Hiroaki Nobuhara, Keisuke Kuida, Makoto Furutani, Toshihiko Shiroishi, Kazuo Moriwaki, Yusuke Yanagi, Tomio Tada

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Southern blots of genomic DNA from 23 strains of laboratory mice and 19 individual wild mice were examined for restriction fragment length polymorphisms in their loci encoding the T-cell receptors (Tcr): the constant regions of the α, β, and γ chains (C α, C β, and C γ) and a variable region family of the β chain (V β8). Only a few polymorphisms were observed for each locus in the laboratory mice after using three restriction enzymes, Bam HI, Eco RI, and Hind III. All the laboratory mice examined fall into one of two types for the C α, C β and V β8 loci and one of three types for the C γ. These types are found in some of the wild mice studied, indicating that they were already present in the founder mice of laboratory mouse strains. In contrast, the Tcr genes are highly polymorphic among wild mice. Analysis of the polymorphisms in these loci suggests that laboratory mice have inherited their genes not only from Mus musculus domesticus, but also from other subspecies, and much more than previously believed from Asian subspecies.

Original languageEnglish
Pages (from-to)405-413
Number of pages9
JournalImmunogenetics
Volume30
Issue number6
DOIs
Publication statusPublished - Dec 1 1989
Externally publishedYes

Fingerprint

T-Cell Receptor Genes
Deoxyribonuclease EcoRI
T-Cell Antigen Receptor
Southern Blotting
Restriction Fragment Length Polymorphisms

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Polymorphism of T-cell receptor genes among laboratory and wild mice : Diverse origins of laboratory mice. / Nobuhara, Hiroaki; Kuida, Keisuke; Furutani, Makoto; Shiroishi, Toshihiko; Moriwaki, Kazuo; Yanagi, Yusuke; Tada, Tomio.

In: Immunogenetics, Vol. 30, No. 6, 01.12.1989, p. 405-413.

Research output: Contribution to journalArticle

Nobuhara, H, Kuida, K, Furutani, M, Shiroishi, T, Moriwaki, K, Yanagi, Y & Tada, T 1989, 'Polymorphism of T-cell receptor genes among laboratory and wild mice: Diverse origins of laboratory mice', Immunogenetics, vol. 30, no. 6, pp. 405-413. https://doi.org/10.1007/BF02421171
Nobuhara, Hiroaki ; Kuida, Keisuke ; Furutani, Makoto ; Shiroishi, Toshihiko ; Moriwaki, Kazuo ; Yanagi, Yusuke ; Tada, Tomio. / Polymorphism of T-cell receptor genes among laboratory and wild mice : Diverse origins of laboratory mice. In: Immunogenetics. 1989 ; Vol. 30, No. 6. pp. 405-413.
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