Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica

Keisuke Okada, Hiroyuki Abe, Fumio Ike, Yoshitoshi Ogura, Tetsuya Hayashi, Aya Fukui-Miyazaki, Keiji Nakamura, Naoaki Shinzawa, Yasuhiko Horiguchi

Research output: Contribution to journalArticle

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Abstract

Bordetella bronchiseptica is a pathogenic bacterium causing respiratory infections in a broad range of mammals. Recently, we determined the whole genome sequence of B. bronchiseptica S798 strain isolated from a pig infected with atrophic rhinitis and found four single-nucleotide polymorphisms (SNPs) at positions -129, -72, +22, and +38 in the region upstream of dnt encoding dermonecrotic toxin (DNT), when compared with a rabbit isolate, RB50. DNT is known to be involved in turbinate atrophy observed in atrophic rhinitis. Immunoblotting, quantitative real-time PCR, and β-galactosidase reporter assay revealed that these SNPs resulted in the increased promoter activity of dnt and conferred the increased ability to produce DNT on the bacteria. Similar or identical SNPs were also found in other pig isolates kept in our laboratory, all of which produce a larger amount of DNT than RB50. Our analysis revealed that substitution of at least two of the four bases, at positions -72 and +22, influenced the promoter activity for dnt. These results imply that these SNPs are involved in the pathogenicity of bordetellae specific to pig diseases.

Original languageEnglish
Article numbere0116604
JournalPloS one
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 2 2015
Externally publishedYes

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Bordetella bronchiseptica
Polymorphism
single nucleotide polymorphism
Single Nucleotide Polymorphism
toxins
Nucleotides
Atrophic Rhinitis
genetic polymorphism
rhinitis
Swine
Bacteria
promoter regions
Galactosidases
galactosidases
Turbinates
swine diseases
swine
Mammals
bacteria
immunoblotting

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica. / Okada, Keisuke; Abe, Hiroyuki; Ike, Fumio; Ogura, Yoshitoshi; Hayashi, Tetsuya; Fukui-Miyazaki, Aya; Nakamura, Keiji; Shinzawa, Naoaki; Horiguchi, Yasuhiko.

In: PloS one, Vol. 10, No. 2, e0116604, 02.02.2015.

Research output: Contribution to journalArticle

Okada, Keisuke ; Abe, Hiroyuki ; Ike, Fumio ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Fukui-Miyazaki, Aya ; Nakamura, Keiji ; Shinzawa, Naoaki ; Horiguchi, Yasuhiko. / Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica. In: PloS one. 2015 ; Vol. 10, No. 2.
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