Poly(N-substituted-acrylamide)-branched schizophyllans are useful for selective recovery of homopolynucleotides through convenient and quick precipitation procedures

Takahiro Matsumoto, Mariko Umeda, Munenori Numata, Teruaki Hasegawa, Kazuo Sakurai, Kazuya Koumoto, Seiji Shinkai

Research output: Contribution to journalArticle

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Abstract

Schizophyllans carrying poly(N-substituted-acrylamidej-branches were synthesized from native schizophyllan through Ce(IV)-initiated radical homo- or co-polymerization of N-iso-propyl- and N-sec-butyl-acryl-amide. A series of structural analyses including elemental analysis, gel permeation chromatography, 1H NMR, and enzymatic degradation using β-1,3-glucanase revealed that schizophyllans (150 kDa) bearing long poly(N-substituted- acrylamide)-branches (ca. 290 kDa) are obtained. These SPG-conjugates form macromolecular complexes with certain homopolynucleotides such as poly(C), poly(dA), and poly(A) and then efficiently co-precipitate them on heating. For example, schizophyllan carrying poly(N-iso-propylacrylamide)-branches can selectively recover poly(C) from a mixture of poly(C) and poly(U) by simple heating (36°C) followed by centrifugation. HPLC analysis of the recovered polynucleotide revealed the high poly(C)-selectivity (poly(C):poly(U) = 95:5). Similarly, schizophyllan carrying more hydrophobic poly(N-iso-propylacrylamide- co-N-sec-butylacrylamide)-branches precipitates at lower temperature (ca. 20°C) and is applicable to isolation of poly(A) that forms less stable macromolecular complexes with SPG and SPG-derivatives (the dissociation temperatures are around 30°C).

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalPolymer Journal
Volume37
Issue number3
DOIs
Publication statusPublished - May 30 2005

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Sizofiran
Acrylamide
Precipitates
Bearings (structural)
Heating
Recovery
Centrifugation
Gel permeation chromatography
Macromolecular Substances
Free radical polymerization
Amides
Copolymerization
Nuclear magnetic resonance
Derivatives
Degradation
Temperature
Polynucleotides
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

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Poly(N-substituted-acrylamide)-branched schizophyllans are useful for selective recovery of homopolynucleotides through convenient and quick precipitation procedures. / Matsumoto, Takahiro; Umeda, Mariko; Numata, Munenori; Hasegawa, Teruaki; Sakurai, Kazuo; Koumoto, Kazuya; Shinkai, Seiji.

In: Polymer Journal, Vol. 37, No. 3, 30.05.2005, p. 177-185.

Research output: Contribution to journalArticle

Matsumoto, Takahiro ; Umeda, Mariko ; Numata, Munenori ; Hasegawa, Teruaki ; Sakurai, Kazuo ; Koumoto, Kazuya ; Shinkai, Seiji. / Poly(N-substituted-acrylamide)-branched schizophyllans are useful for selective recovery of homopolynucleotides through convenient and quick precipitation procedures. In: Polymer Journal. 2005 ; Vol. 37, No. 3. pp. 177-185.
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