Polyol metabolism in the mycelium and fruit-bodies during development of Flammulina velutipes

Y. Kitamoto, A. Kikuchi, N. Mori, Shoji Ohga

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Changes of polyol contents in the mycelium and fruit-bodies of Flammulina velutipes were measured. The results suggested that arabinitol is accumulated in the fruit-bodies as the end-product after its translocation from the mycelium, while mannitol in the fruit-bodies is converted into fructose by the action of mannitol dehydrogenase (MDH). The development of fruit-bodies was promoted by feeding of mannitol to the mycelial colony. A 14C tracer experiment indicated that half of mannitol translocated from mycelium to fruit-bodies was utilized for fruit-body development. NAD-linked MDH and D-arabinitol dehydrogenase (D-ADH) were detected in both mycelium and fruit-bodies. The activities of MDH and ADH in the mycelium reached their maximum levels in the initial stage of fruit-body development and decreased thereafter. In contrast, the activity of MDH in the fruit-bodies showed a peak in the middle stage of development. The activity of ADH in the fruit-bodies was less than half of that of MDH. MDH showed a lower Km value for mannitol (1.3 × 10-3 M) than for fructose (6.0 × 10-2 M). The Km value of ADH for arabinitol was extremely high (1.3 × 10-1 M).

Original languageEnglish
Pages (from-to)461-465
Number of pages5
JournalMycoscience
Volume41
Issue number5
Publication statusPublished - Oct 1 2000

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Flammulina velutipes
polyols
fruiting bodies
mannitol
mycelium
fruit
metabolism
arabinitol
fructose
translocation
tracer techniques
tracer

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

Cite this

Kitamoto, Y., Kikuchi, A., Mori, N., & Ohga, S. (2000). Polyol metabolism in the mycelium and fruit-bodies during development of Flammulina velutipes. Mycoscience, 41(5), 461-465.

Polyol metabolism in the mycelium and fruit-bodies during development of Flammulina velutipes. / Kitamoto, Y.; Kikuchi, A.; Mori, N.; Ohga, Shoji.

In: Mycoscience, Vol. 41, No. 5, 01.10.2000, p. 461-465.

Research output: Contribution to journalArticle

Kitamoto, Y, Kikuchi, A, Mori, N & Ohga, S 2000, 'Polyol metabolism in the mycelium and fruit-bodies during development of Flammulina velutipes', Mycoscience, vol. 41, no. 5, pp. 461-465.
Kitamoto, Y. ; Kikuchi, A. ; Mori, N. ; Ohga, Shoji. / Polyol metabolism in the mycelium and fruit-bodies during development of Flammulina velutipes. In: Mycoscience. 2000 ; Vol. 41, No. 5. pp. 461-465.
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