Polyol metabolism in the mycelium and fruit-bodies during development of Flammulina velutipes

Y. Kitamoto, A. Kikuchi, N. Mori, S. Ohga

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Changes of polyol contents in the mycelium and fruit-bodies of Flammulina velutipes were measured. The results suggested that arabinitol is accumulated in the fruit-bodies as the end-product after its translocation from the mycelium, while mannitol in the fruit-bodies is converted into fructose by the action of mannitol dehydrogenase (MDH). The development of fruit-bodies was promoted by feeding of mannitol to the mycelial colony. A 14C tracer experiment indicated that half of mannitol translocated from mycelium to fruit-bodies was utilized for fruit-body development. NAD-linked MDH and D-arabinitol dehydrogenase (D-ADH) were detected in both mycelium and fruit-bodies. The activities of MDH and ADH in the mycelium reached their maximum levels in the initial stage of fruit-body development and decreased thereafter. In contrast, the activity of MDH in the fruit-bodies showed a peak in the middle stage of development. The activity of ADH in the fruit-bodies was less than half of that of MDH. MDH showed a lower Km value for mannitol (1.3 × 10-3 M) than for fructose (6.0 × 10-2 M). The Km value of ADH for arabinitol was extremely high (1.3 × 10-1 M).

Original languageEnglish
Pages (from-to)461-465
Number of pages5
JournalMycoscience
Volume41
Issue number5
Publication statusPublished - Oct 2000

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

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