Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column

Taro Kimura, Kazuya Koumoto, Masami Mizu, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.

Original languageEnglish
Pages (from-to)1240-1241
Number of pages2
JournalChemistry Letters
Issue number12
DOIs
Publication statusPublished - Dec 5 2002

Fingerprint

Sizofiran
Polynucleotides
Polysaccharides
Poly G
RNA
Poly A
Glucans
Biomolecules
Complexation

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column. / Kimura, Taro; Koumoto, Kazuya; Mizu, Masami; Sakurai, Kazuo; Shinkai, Seiji.

In: Chemistry Letters, No. 12, 05.12.2002, p. 1240-1241.

Research output: Contribution to journalArticle

Kimura, Taro ; Koumoto, Kazuya ; Mizu, Masami ; Sakurai, Kazuo ; Shinkai, Seiji. / Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column. In: Chemistry Letters. 2002 ; No. 12. pp. 1240-1241.
@article{1b0f854c4861457aaed21c66d629cd75,
title = "Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column",
abstract = "Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.",
author = "Taro Kimura and Kazuya Koumoto and Masami Mizu and Kazuo Sakurai and Seiji Shinkai",
year = "2002",
month = "12",
day = "5",
doi = "10.1246/cl.2002.1240",
language = "English",
pages = "1240--1241",
journal = "Chemistry Letters",
issn = "0366-7022",
publisher = "The Chemical Society of Japan",
number = "12",

}

TY - JOUR

T1 - Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column

AU - Kimura, Taro

AU - Koumoto, Kazuya

AU - Mizu, Masami

AU - Sakurai, Kazuo

AU - Shinkai, Seiji

PY - 2002/12/5

Y1 - 2002/12/5

N2 - Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.

AB - Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.

UR - http://www.scopus.com/inward/record.url?scp=0037028349&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037028349&partnerID=8YFLogxK

U2 - 10.1246/cl.2002.1240

DO - 10.1246/cl.2002.1240

M3 - Article

AN - SCOPUS:0037028349

SP - 1240

EP - 1241

JO - Chemistry Letters

JF - Chemistry Letters

SN - 0366-7022

IS - 12

ER -