Polysaccharide schizophyllan-based plasm id DNA carrier

T. Nagasaki, T. Satoh, A. Uno, M. Hojo, K. Koumoto, M. Mizu, K. Sakurai, Seiji Shinkai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of polysaccharide schizophyllan-based plasmid DNA carrier was analyzed. Amine-introduced cationic schizophyllan (SPG) carriers were prepared using ethanolamine, spermidine, spermine, and N,N'-bis(3-aminopropyl)- 1,3-propanediamine. It was found that transfection efficiency was dependent on cation/anion ratios, with carriers having SPG as the backbone showing the highest transfection efficiency with C/A ratios of 10-15. It was observed that transfection efficiency of the 80,000 Da derivative was about eight times greater than that of PEI.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - Dec 1 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period5/17/045/21/04

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Polysaccharides
DNA
Ethanolamines
Polyetherimides
Amines
Negative ions
Positive ions
Derivatives

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Nagasaki, T., Satoh, T., Uno, A., Hojo, M., Koumoto, K., Mizu, M., ... Shinkai, S. (2004). Polysaccharide schizophyllan-based plasm id DNA carrier. In Transactions - 7th World Biomaterials Congress

Polysaccharide schizophyllan-based plasm id DNA carrier. / Nagasaki, T.; Satoh, T.; Uno, A.; Hojo, M.; Koumoto, K.; Mizu, M.; Sakurai, K.; Shinkai, Seiji.

Transactions - 7th World Biomaterials Congress. 2004.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nagasaki, T, Satoh, T, Uno, A, Hojo, M, Koumoto, K, Mizu, M, Sakurai, K & Shinkai, S 2004, Polysaccharide schizophyllan-based plasm id DNA carrier. in Transactions - 7th World Biomaterials Congress. Transactions - 7th World Biomaterials Congress, Sydney, Australia, 5/17/04.
Nagasaki T, Satoh T, Uno A, Hojo M, Koumoto K, Mizu M et al. Polysaccharide schizophyllan-based plasm id DNA carrier. In Transactions - 7th World Biomaterials Congress. 2004
Nagasaki, T. ; Satoh, T. ; Uno, A. ; Hojo, M. ; Koumoto, K. ; Mizu, M. ; Sakurai, K. ; Shinkai, Seiji. / Polysaccharide schizophyllan-based plasm id DNA carrier. Transactions - 7th World Biomaterials Congress. 2004.
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AU - Sakurai, K.

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