Polysaccharide schizophyllan-based plasm id DNA carrier

T. Nagasaki, T. Satoh, A. Uno, M. Hojo, K. Koumoto, M. Mizu, K. Sakurai, S. Shinkai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of polysaccharide schizophyllan-based plasmid DNA carrier was analyzed. Amine-introduced cationic schizophyllan (SPG) carriers were prepared using ethanolamine, spermidine, spermine, and N,N'-bis(3-aminopropyl)- 1,3-propanediamine. It was found that transfection efficiency was dependent on cation/anion ratios, with carriers having SPG as the backbone showing the highest transfection efficiency with C/A ratios of 10-15. It was observed that transfection efficiency of the 80,000 Da derivative was about eight times greater than that of PEI.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - Dec 1 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period5/17/045/21/04

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Nagasaki, T., Satoh, T., Uno, A., Hojo, M., Koumoto, K., Mizu, M., ... Shinkai, S. (2004). Polysaccharide schizophyllan-based plasm id DNA carrier. In Transactions - 7th World Biomaterials Congress (Transactions - 7th World Biomaterials Congress).