Abstract
Recently, we found that schizophyllan (SPG), which is a natural and neutral polysaccharide, can form macromolecular complexes with certain polynucleotides. In order to increase the stability and the affinity of the complexes, we synthesized a 2-aminoethanol-appended SPG (N-s-SPG) by utilizing periodate oxidation and reductive amination. Although the modification level was only 2.4 ± 0.3%, the melting temperature of the poly(C)–N-s-SPG complex was increased by 8 °C comparing with the poly(C)–s-SPG complex. In addition, the conformation of the complex was scarcely perturbed by the introduction of amino groups. When we formed complexes of N-s-SPG and other polynucleotides, the melting temperature of their complexes increased without exception. Moreover, the amino modification induced complexation with poly(U), which does not form a complex with SPG. The affinity for the short chain nucleotide was also enhanced. These results indicate that the present modification method is quite useful to improve complex stability and we believe, therefore, that this strategy will make it possible to apply SPG to a novel gene carrier.
| Original language | English |
|---|---|
| Pages (from-to) | 2477-2484 |
| Number of pages | 8 |
| Journal | Journal of the Chemical Society. Perkin Transactions 1 |
| Volume | 2 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - Aug 1 2002 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Polysaccharide–polynucleotide complexes. / Koumoto, Kazuya; Kimura, Taro; Mizu, Masami; Kunitake, Toyoki; Sakurai, Kazuo; Shinkai, Seiji.
In: Journal of the Chemical Society. Perkin Transactions 1, Vol. 2, No. 22, 01.08.2002, p. 2477-2484.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polysaccharide–polynucleotide complexes
AU - Koumoto, Kazuya
AU - Kimura, Taro
AU - Mizu, Masami
AU - Kunitake, Toyoki
AU - Sakurai, Kazuo
AU - Shinkai, Seiji
PY - 2002/8/1
Y1 - 2002/8/1
N2 - Recently, we found that schizophyllan (SPG), which is a natural and neutral polysaccharide, can form macromolecular complexes with certain polynucleotides. In order to increase the stability and the affinity of the complexes, we synthesized a 2-aminoethanol-appended SPG (N-s-SPG) by utilizing periodate oxidation and reductive amination. Although the modification level was only 2.4 ± 0.3%, the melting temperature of the poly(C)–N-s-SPG complex was increased by 8 °C comparing with the poly(C)–s-SPG complex. In addition, the conformation of the complex was scarcely perturbed by the introduction of amino groups. When we formed complexes of N-s-SPG and other polynucleotides, the melting temperature of their complexes increased without exception. Moreover, the amino modification induced complexation with poly(U), which does not form a complex with SPG. The affinity for the short chain nucleotide was also enhanced. These results indicate that the present modification method is quite useful to improve complex stability and we believe, therefore, that this strategy will make it possible to apply SPG to a novel gene carrier.
AB - Recently, we found that schizophyllan (SPG), which is a natural and neutral polysaccharide, can form macromolecular complexes with certain polynucleotides. In order to increase the stability and the affinity of the complexes, we synthesized a 2-aminoethanol-appended SPG (N-s-SPG) by utilizing periodate oxidation and reductive amination. Although the modification level was only 2.4 ± 0.3%, the melting temperature of the poly(C)–N-s-SPG complex was increased by 8 °C comparing with the poly(C)–s-SPG complex. In addition, the conformation of the complex was scarcely perturbed by the introduction of amino groups. When we formed complexes of N-s-SPG and other polynucleotides, the melting temperature of their complexes increased without exception. Moreover, the amino modification induced complexation with poly(U), which does not form a complex with SPG. The affinity for the short chain nucleotide was also enhanced. These results indicate that the present modification method is quite useful to improve complex stability and we believe, therefore, that this strategy will make it possible to apply SPG to a novel gene carrier.
UR - http://www.scopus.com/inward/record.url?scp=0036436468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036436468&partnerID=8YFLogxK
U2 - 10.1039/b207553a
DO - 10.1039/b207553a
M3 - Article
AN - SCOPUS:0036436468
VL - 2
SP - 2477
EP - 2484
JO - Journal of the Chemical Society, Perkin Transactions 2
JF - Journal of the Chemical Society, Perkin Transactions 2
SN - 1472-7781
IS - 22
ER -