Polysaccharide/polynucleotide complexes Part 6: Complementary-strand- induced release of single-stranded DNA bound in the schizophyllan complex

Kazuya Koumoto, Masami Mizu, Kazuo Sakurai, Toyoki Kunitake, Seiji Shinkai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA · 2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA · 2s-SPG induced the quickreleas e of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.

Original languageEnglish
Pages (from-to)520-529
Number of pages10
JournalChemistry and Biodiversity
Volume1
Issue number3
DOIs
Publication statusPublished - Jan 1 2004

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Molecular Medicine
  • Molecular Biology

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